Comparison and Evaluation of Abbott Chemiluminescent Microparticle Immunoassay and ChIVD Light-initiated Chemiluminescent Assay in the Detection of Treponema Pallidum Antibody

Overview

Journal J Clin Lab Anal

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
Pathology

Date 2020 Mar 6

PMID 32133697

Citations 2

Authors

Xiaohui Chen

Ruifeng Yang

Yongming Liang

Teng Yuan

Jiansuo Zhou

Tiancheng Wang

Liyan Cui

Affiliations

Soon will be listed here.

Abstract

Background: Laboratory tests play an important role in the diagnosis of syphilis. This study aimed to compare and assess the performance of the Abbott chemiluminescent microparticle immunoassay (CMIA) and the ChIVD light-initiated chemiluminescent assay (LICA) in the detection of Treponema pallidum (TP) antibody.

Methods: A total of 10 498 serum samples were detected with two assays, and the Treponema pallidum particle agglutination assay (TPPA) and recombinant immunoblot assay (RIBA) methods were used for confirmation. The sensitivity, specificity, positive predictive value, and negative predictive value of the Abbott CMIA and ChIVD LICA were calculated. The coincidence rate between two assays was also evaluated. The causes of false positive and false negative of two assays were studied.

Results: For the Abbott CMIA and ChIVD LICA, the sensitivity was 94.44% and 98.15%, the specificity was 99.89% and 99.81%, the positive predictive value was 93.29% and 88.83%, and the negative predictive value was 99.91% and 99.97%, respectively. The coincidence rate between Abbott CMIA and ChIVD LICA was 99.26%, and κ value was .790. The disease of infertility, hypertensive disease, liver disease, and cancer were the common causes of false positive in both assays, while infertility was also the main reason lead to false negative.

Conclusion: Our results demonstrated that the Abbott CMIA and ChIVD LICA generally had high sensitivity and specificity and therefore may be suitable for the detection of TP antibody and screening for syphilis.

Citing Articles

Assessment of recombinant antigens Tp0100 and Tp1016 of Treponema pallidum for serological diagnosis of syphilis.

Chen D, Wang S, He Y, Fu Y, Zhao F, Zhou X J Clin Lab Anal. 2022; 36(9):e24635.

PMID: 35908795 PMC: 9459255. DOI: 10.1002/jcla.24635.

Comparison and evaluation of Abbott chemiluminescent microparticle immunoassay and ChIVD light-initiated chemiluminescent assay in the detection of Treponema pallidum antibody.

Chen X, Yang R, Liang Y, Yuan T, Zhou J, Wang T J Clin Lab Anal. 2020; 34(7):e23275.

PMID: 32133697 PMC: 7370704. DOI: 10.1002/jcla.23275.

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