The Effect of Ellagic Acid on Caspase-3/bcl-2/Nrf-2/NF-kB/TNF-α /COX-2 Gene Expression Product Apoptosis Pathway: a New Approach for Muscle Damage Therapy

Overview

Journal Mol Biol Rep

Specialty Molecular Biology

Date 2020 Mar 4

PMID 32124171

Citations 10

Authors

Abdullah Aslan

Seda Beyaz

Ozlem Gok

Orhan Erman

Affiliations

Soon will be listed here.

Abstract

The goal of this study was to determine the protective role of ellagic acid (EA) against CCl-induced muscle injury in rats. In this study, 36 Wistar albino rats (n = 36, 8 weeks old) were used. The rats were divided into 4 groups and 9 rats were included each group. Groups: (i) control Group: standard diet; (ii) EA Group: standard diet + EA group; (iii) CCl group: standard diet + CCl group; (iv) EA + CCl group: standard diet + EA + CCl. The animals were decapitated after 8 weeks, and their muscle tissues were received and investigated. In the muscle tissue, TNF-α, COX-2, Nrf-2, NF-kB, caspase-3 and bcl-2 expression levels were analyzed by the western blotting technique, lipid peroxidation was detected by MDA (malondialdehyde), and catalase and GSH levels were determined by a spectrophotometer. In our findings, in comparison to the CCl group, in the EA + CCl group, the Nrf-2 and caspase-3 protein expression levels, GSH and catalase activities increased, while the NF-kB, bcl-2, TNF-α and COX-2 protein expression levels and MDA levels decreased. These results suggest that EA reduces muscle tissue damage rate in rats and that EA may also be used as a potential drug to protect against muscle tissue damage in the future.

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