Effects of Grape Seed Proanthocyanidin Extract on Side Effects of High-dose Methylprednisolone Administration in Male Rats

Overview

Journal Toxicol Res

Specialty Toxicology

Date 2023 Oct 2

PMID 37779581

Authors

Aslihan Sur

Seda Iflazoglu Mutlu

Pinar Tatli Seven

Ismail Seven

Abdullah Aslan

Meltem Kizil

Recai Kulaksiz

Mustafa Hilmi Yaranoglu

Selim Esen

Affiliations

Soon will be listed here.

Abstract

In this study, we investigated the effects of grape seed proanthocyanidin extract (GSPE) against the side effects of high-dose administration of methylprednisolone (MP) in male rats. A total of 32 adult Wistar male albino rats were divided into four groups: (1) control (CON), received standard food only; (2) MP, received standard food + intraperitoneal injection of 60 mg/kg MP on day 7; (3) GSPE, received standard food + 200 mg/kg/day GSPE; and (4) MP + GSPE, received standard food + 200 mg/kg/day of GSPE + intraperitoneal injection of 60 mg/kg MP on day 7. All animals in the GSPE and GSPE + MP groups were treated once a day by oral gavage for 14 consecutive days. The feed intake of rats in the MP and MP + GSPE groups decreased significantly by 24.14% and 13.52%, respectively ( < 0.05). Administration of MP resulted in significant increases in serum concentrations of blood urea nitrogen ( < 0.001), glucose ( < 0.01), alkaline phosphatase, and adrenocorticotropic hormone ( < 0.05). High-dose MP administration significantly reduced catalase ( < 0.001) and glutathione peroxidase ( < 0.05) concentrations in the liver and kidney tissues of rats, while glutathione concentrations were only reduced in liver tissue ( < 0.05). The expression levels of and in liver, kidney, and testicular tissue were significantly increased, while the expression levels of were reduced ( < 0.001). Furthermore, sperm concentration was significantly affected by GSPE in rats induced by high-dose MP, and sperm loss was significantly reduced in MP + GSPE ( < 0.05). These findings suggest that GSPE could be useful as a supplement to alleviate MP-induced toxicity in rats.

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