Photoinactivation of Moraxella Catarrhalis Using 405-nm Blue Light: Implications for the Treatment of Otitis Media

Overview

Journal Photochem Photobiol

Specialties Biochemistry
Biology
Chemistry

Date 2020 Feb 28

PMID 32105346

Citations 8

Authors

Xiaojing Liu

Qihang Chang

Raquel Ferrer-Espada

Leon G Leanse

Xueping Sharon Goh

Xiuli Wang

Jeffrey A Gelfand

Tianhong Dai

Affiliations

Soon will be listed here.

Abstract

Moraxella catarrhalis is one of the major otopathogens of otitis media (OM) in childhood. M. catarrhalis tends to form biofilm, which contributes to the chronicity and recurrence of infections, as well as resistance to antibiotic treatment. In this study, we aimed to investigate the effectiveness of antimicrobial blue light (aBL; 405 nm), an innovative nonpharmacological approach, for the inactivation of M. catarrhalis OM. M. catarrhalis either in planktonic suspensions or 24-h old biofilms were exposed to aBL at the irradiance of 60 mW cm . Under an aBL exposure of 216 J cm , a >4-log colony-forming units (CFU) reduction in planktonic suspensions and a >3-log CFU reduction in biofilms were observed. Both transmission electron microscopy and scanning electron microscopy revealed aBL-induced morphological damage in M. catarrhalis. Ultraperformance liquid chromatography results indicated that protoporphyrin IX and coproporphyrin were the two most abundant species of endogenous photosensitizing porphyrins. No statistically significant reduction in the viability of HaCaT cells was observed after an aBL exposure of up to 216 J cm . Collectively, our results suggest that aBL is potentially an effective and safe alternative therapy for OM caused by M. catarrhalis. Further in vivo studies are warranted before this optical approach can be moved to the clinics.

Citing Articles

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