MicroRNA-20b-5p Regulates Propofol-preconditioning-induced Inhibition of Autophagy in Hypoxia-and-reoxygenation-stimulated Endothelial Cells

Overview

Journal J Biosci

Specialties Biochemistry
Biology

Date 2020 Feb 27

PMID 32098914

Citations 10

Authors

Wang Zhen

Ding Hui

Song Wenying

Song Yulong

Affiliations

Soon will be listed here.

Abstract

Ischemia-reperfusion (IR) injury is a major cause of clinical emergencies during and after surgical procedures. Propofol protects the heart from cardiovascular IR injury by inhibiting autophagy. MicroRNAs (miRNAs) participate in anesthetic-regulated cardiovascular injury. MiR-20b-5p targets unc-51-like autophagy activating kinase 1 (ULK1). Its role in propofol-modulated cardiovascular IR injury remains unclear, however. In this study, we used an model of hypoxia-reoxygenation (HR)-induced injury to human umbilical vein endothelial cells (HUVECs) to determine the protective effect of miR-20b-5p in cells preconditioned with propofol. We found that miR-20b-5p was significantly higher and ULK1 was lower in propofol-preconditioned HUVECs with HR injury than in HUVECs with HR injury only. Additionally, miR-20b-5p overexpression increased cell viability and repressed autophagy and apoptosis more in propofol-preconditioned HUVECs with HR injury than in HUVECs with HR injury only. A luciferase reporter assay confirmed the target reaction between miR-20b-5p and ULK1. Overexpression of ULK1 restrained the protective effect of miR-20b-5p in propofol-preconditioned HUVECs with HR injury. In conclusion, our results indicate that propofol inhibits autophagic cell death via the miR-20b-5p-ULKI axis and that ULK1 may be a therapeutic target for cardiovascular IR injury.

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References

Chen C, Wu C, Shih C, Lien W, Huang S, Huang C . Attenuation of Isoflurane Preconditioning-Induced Acute Cardioprotection in Hypertensive Hypertrophied Hearts. J Cardiothorac Vasc Anesth. 2016; 30(5):1317-23. DOI: 10.1053/j.jvca.2016.03.131. View

Chen Z, Hu Z, Lu Z, Cai S, Gu X, Zhuang H . Differential microRNA profiling in a cellular hypoxia reoxygenation model upon posthypoxic propofol treatment reveals alterations in autophagy signaling network. Oxid Med Cell Longev. 2014; 2013:378484. PMC: 3885199. DOI: 10.1155/2013/378484. View

Huang W, Wen J, Lin R, Wei P, Huang F . Effects of mTOR/NF-κB signaling pathway and high thoracic epidural anesthesia on myocardial ischemia-reperfusion injury via autophagy in rats. J Cell Physiol. 2017; 233(9):6669-6678. DOI: 10.1002/jcp.26320. View

Sukseree S, Rossiter H, Mildner M, Pammer J, Buchberger M, Gruber F . Targeted deletion of Atg5 reveals differential roles of autophagy in keratin K5-expressing epithelia. Biochem Biophys Res Commun. 2012; 430(2):689-94. DOI: 10.1016/j.bbrc.2012.11.090. View

Ghavami S, Gupta S, Ambrose E, Hnatowich M, Freed D, Dixon I . Autophagy and heart disease: implications for cardiac ischemia-reperfusion damage. Curr Mol Med. 2014; 14(5):616-29. DOI: 10.2174/1566524014666140603101520. View

Harman F, Hasturk A, Yaman M, Arca T, Kilinc K, Sargon M . Neuroprotective effects of propofol, thiopental, etomidate, and midazolam in fetal rat brain in ischemia-reperfusion model. Childs Nerv Syst. 2012; 28(7):1055-62. DOI: 10.1007/s00381-012-1782-0. View

Liu Q, Wu D, Han L, Deng J, Zhou L, He R . Roles of microRNAs in psoriasis: Immunological functions and potential biomarkers. Exp Dermatol. 2016; 26(4):359-367. PMC: 5837862. DOI: 10.1111/exd.13249. View

Sun T, Li M, Li P, Cao J . MicroRNAs in Cardiac Autophagy: Small Molecules and Big Role. Cells. 2018; 7(8). PMC: 6116024. DOI: 10.3390/cells7080104. View

Liu X, Xu T, Hu X, Chen X, Zeng K, Sun L . Elevated circulating miR-182 acts as a diagnostic biomarker for early colorectal cancer. Cancer Manag Res. 2018; 10:857-865. PMC: 5927061. DOI: 10.2147/CMAR.S158016. View

10.

Ye F, Zuo Z . Anesthetic effects on autophagy. Med Gas Res. 2017; 7(3):204-211. PMC: 5674659. DOI: 10.4103/2045-9912.215751. View

11.

Parzych K, Klionsky D . An overview of autophagy: morphology, mechanism, and regulation. Antioxid Redox Signal. 2013; 20(3):460-73. PMC: 3894687. DOI: 10.1089/ars.2013.5371. View

12.

Zhou H, Yu Y, Zhang J, Zhang Y, Luan Q, Wang G . Protective Effects the Akt Activator SC79 in Hepatic Ischemia-Reperfusion Injury. Med Sci Monit. 2018; 24:4346-4354. PMC: 6049012. DOI: 10.12659/MSM.911178. View

13.

Luengo-Gil G, Gonzalez-Billalabeitia E, Perez-Henarejos S, Navarro Manzano E, Chaves-Benito A, Garcia-Martinez E . Angiogenic role of miR-20a in breast cancer. PLoS One. 2018; 13(4):e0194638. PMC: 5884522. DOI: 10.1371/journal.pone.0194638. View

14.

Yoshii S, Mizushima N . Monitoring and Measuring Autophagy. Int J Mol Sci. 2017; 18(9). PMC: 5618514. DOI: 10.3390/ijms18091865. View

15.

Chang C, Chen P, Lu S, Hsieh M, Lin C, Lee H . Propofol-enhanced autophagy increases motility and angiogenic capacity of cultured human umbilical vascular endothelial cells. Life Sci. 2015; 142:49-59. DOI: 10.1016/j.lfs.2015.10.014. View

16.

Chidambaran V, Costandi A, DMello A . Propofol: a review of its role in pediatric anesthesia and sedation. CNS Drugs. 2015; 29(7):543-63. PMC: 4554966. DOI: 10.1007/s40263-015-0259-6. View

17.

Soro M, Gallego L, Silva V, Ballester M, Llorens J, Alvarino A . Cardioprotective effect of sevoflurane and propofol during anaesthesia and the postoperative period in coronary bypass graft surgery: a double-blind randomised study. Eur J Anaesthesiol. 2012; 29(12):561-9. DOI: 10.1097/EJA.0b013e3283560aea. View

18.

Hafren A, Macia J, Love A, Milner J, Drucker M, Hofius D . Selective autophagy limits cauliflower mosaic virus infection by NBR1-mediated targeting of viral capsid protein and particles. Proc Natl Acad Sci U S A. 2017; 114(10):E2026-E2035. PMC: 5347569. DOI: 10.1073/pnas.1610687114. View

19.

Perricone A, Vander Heide R . Novel therapeutic strategies for ischemic heart disease. Pharmacol Res. 2014; 89:36-45. PMC: 4182165. DOI: 10.1016/j.phrs.2014.08.004. View

20.

Liang Z, Yao H, Xie R, Gong C, Tian Y . MicroRNA‑20b‑5p promotes ventricular remodeling by targeting the TGF‑β/Smad signaling pathway in a rat model of ischemia‑reperfusion injury. Int J Mol Med. 2018; 42(2):975-987. PMC: 6034914. DOI: 10.3892/ijmm.2018.3695. View