Promoter Polymorphisms in the LncRNA-MIAT Gene Associated with Acute Myocardial Infarction in Chinese Han Population: a Case-control Study

Overview

Journal Biosci Rep

Specialty Cell Biology

Date 2020 Feb 25

PMID 32090249

Citations 7

Authors

Ruchao Ma

Xiaohui He

Xiaoyun Zhu

Shuchao Pang

Bo Yan

Affiliations

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Abstract

Background: Coronary atherosclerotic disease (CAD) is one of the greatest causes of death and disability around the world, and has emerged as a major public health problem. Acute myocardial infarction (AMI) is the most serious type of CAD. Myocardial infarction (MI) association transcript (MIAT) has demonstrated that it plays an important role in AMI.

Purpose: To investigate the association between MIAT promoter polymorphisms and AMI in Chinese Han population.

Methods: A total of 212 AMI patients and 218 healthy controls were recruited. The long non-coding RNA (lncRNA)-MIAT promoter polymorphisms (single nucleotide polymorphisms (SNPs)) were obtained using polymerase chain reaction (PCR) and sequencing techniques. Chi-square test was used to analyze the allele and genotype frequencies of each SNP in two groups. Logistic regression analysis was used to analyze the association of each SNP with AMI. Linkage disequilibrium (LD) and haplotype analysis were performed using SHEsis software. A JASPAR database search predicts transcription factors transition of linked polymorphism in MIAT promoter.

Results: Ten SNPs were found, including rs56371714, rs55892869, rs151057042, rs2157598, rs150465374, rs5761664, rs8142890, rs5752375, rs9608515 and rs1055293700, whereas rs1055293700 was found only in the control group. Single and logistic regression analysis showed that there was a significant correlation between rs5752375 and rs9608515 polymorphisms and AMI, while other sites had no relationship with AMI. These MI association polymorphisms may change the binding sites with transcription factor.

Conclusions: The polymorphisms of lncRNA-MIAT promoter rs5752375 and rs9608515 were significantly associated with AMI in Chinese Han population. This result would be of clinical importance for the early diagnosis of AMI.

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References

Arnett D, Baird A, Barkley R, Basson C, Boerwinkle E, Ganesh S . Relevance of genetics and genomics for prevention and treatment of cardiovascular disease: a scientific statement from the American Heart Association Council on Epidemiology and Prevention, the Stroke Council, and the Functional Genomics and.... Circulation. 2007; 115(22):2878-901. DOI: 10.1161/CIRCULATIONAHA.107.183679. View

Rao S, Hu H, Ye N, Shen Y, Xu Q . Genetic variants in long non-coding RNA MIAT contribute to risk of paranoid schizophrenia in a Chinese Han population. Schizophr Res. 2015; 166(1-3):125-30. DOI: 10.1016/j.schres.2015.04.032. View

Hu L, Su G, Wang X . The roles of ANRIL polymorphisms in coronary artery disease: a meta-analysis. Biosci Rep. 2019; 39(12). PMC: 6923330. DOI: 10.1042/BSR20181559. View

Ye Z, Yang S, Xia Y, Hu R, Chen S, Li B . LncRNA MIAT sponges miR-149-5p to inhibit efferocytosis in advanced atherosclerosis through CD47 upregulation. Cell Death Dis. 2019; 10(2):138. PMC: 6372637. DOI: 10.1038/s41419-019-1409-4. View

Khan A, Fornes O, Stigliani A, Gheorghe M, Castro-Mondragon J, van der Lee R . JASPAR 2018: update of the open-access database of transcription factor binding profiles and its web framework. Nucleic Acids Res. 2017; 46(D1):D260-D266. PMC: 5753243. DOI: 10.1093/nar/gkx1126. View

Hu Y, Guo F, Xu Y, Li P, Lu Z, McVey D . Long noncoding RNA NEXN-AS1 mitigates atherosclerosis by regulating the actin-binding protein NEXN. J Clin Invest. 2018; 129(3):1115-1128. PMC: 6391138. DOI: 10.1172/JCI98230. View

Go G . Low-Density Lipoprotein Receptor-Related Protein 6 (LRP6) Is a Novel Nutritional Therapeutic Target for Hyperlipidemia, Non-Alcoholic Fatty Liver Disease, and Atherosclerosis. Nutrients. 2015; 7(6):4453-64. PMC: 4488795. DOI: 10.3390/nu7064453. View

Barry G, Briggs J, Vanichkina D, Poth E, Beveridge N, Ratnu V . The long non-coding RNA Gomafu is acutely regulated in response to neuronal activation and involved in schizophrenia-associated alternative splicing. Mol Psychiatry. 2013; 19(4):486-94. DOI: 10.1038/mp.2013.45. View

Ji H, Zhou C, Pan R, Han L, Chen W, Xu X . APOE hypermethylation is significantly associated with coronary heart disease in males. Gene. 2018; 689:84-89. DOI: 10.1016/j.gene.2018.11.088. View

10.

Vausort M, Wagner D, Devaux Y . Long noncoding RNAs in patients with acute myocardial infarction. Circ Res. 2014; 115(7):668-77. DOI: 10.1161/CIRCRESAHA.115.303836. View

11.

Wilson P, DAgostino R, Levy D, Belanger A, Silbershatz H, Kannel W . Prediction of coronary heart disease using risk factor categories. Circulation. 1998; 97(18):1837-47. DOI: 10.1161/01.cir.97.18.1837. View

12.

Arslan S, Berkan O, Lalem T, Ozbilum N, Goksel S, Korkmaz O . Long non-coding RNAs in the atherosclerotic plaque. Atherosclerosis. 2017; 266:176-181. DOI: 10.1016/j.atherosclerosis.2017.10.012. View

13.

Shoeibi S, Mozdziak P, Mohammadi S . Important signals regulating coronary artery angiogenesis. Microvasc Res. 2017; 117:1-9. DOI: 10.1016/j.mvr.2017.12.002. View

14.

Yan B, Yao J, Liu J, Li X, Wang X, Li Y . lncRNA-MIAT regulates microvascular dysfunction by functioning as a competing endogenous RNA. Circ Res. 2015; 116(7):1143-56. DOI: 10.1161/CIRCRESAHA.116.305510. View

15.

Crea F, Venalainen E, Ci X, Cheng H, Pikor L, Parolia A . The role of epigenetics and long noncoding RNA MIAT in neuroendocrine prostate cancer. Epigenomics. 2016; 8(5):721-31. DOI: 10.2217/epi.16.6. View

16.

Boon R, Jae N, Holdt L, Dimmeler S . Long Noncoding RNAs: From Clinical Genetics to Therapeutic Targets?. J Am Coll Cardiol. 2016; 67(10):1214-1226. DOI: 10.1016/j.jacc.2015.12.051. View

17.

Li Y, Wang J, Sun L, Zhu S . RETRACTED: LncRNA myocardial infarction-associated transcript (MIAT) contributed to cardiac hypertrophy by regulating TLR4 via miR-93. Eur J Pharmacol. 2017; 818:508-517. DOI: 10.1016/j.ejphar.2017.11.031. View

18.

Panning B, Jaenisch R . RNA and the epigenetic regulation of X chromosome inactivation. Cell. 1998; 93(3):305-8. DOI: 10.1016/s0092-8674(00)81155-1. View

19.

Jae N, Dimmeler S . Long noncoding RNAs in diabetic retinopathy. Circ Res. 2015; 116(7):1104-6. DOI: 10.1161/CIRCRESAHA.115.306051. View

20.

Wang Y, Song X, Li Z, Liu B . Long non-coding RNAs in coronary atherosclerosis. Life Sci. 2018; 211:189-197. DOI: 10.1016/j.lfs.2018.08.072. View