Association of Angio-LncRNAs MIAT Rs1061540/MALAT1 Rs3200401 Molecular Variants with Gensini Score in Coronary Artery Disease Patients Undergoing Angiography

Overview

Journal Biomolecules

Publisher MDPI

Specialties Biochemistry
Molecular Biology

Date 2022 Jan 21

PMID 35053285

Authors

Mohamed Y Elwazir

Mohammad H Hussein

Eman A Toraih

Essam Al Ageeli

Safya E Esmaeel

Manal S Fawzy

Salwa Faisal

Affiliations

Soon will be listed here.

Abstract

Long non-coding RNAs (lncRNAs) have emerged as essential biomolecules with variable diagnostic and/or prognostic utility in several diseases, including coronary artery disease (CAD). We aimed for the first time to investigate the potential association of five angiogenesis-related lncRNAs (PUNISHER, SENCR, MIAT, MALAT1, and GATA6-AS) variants with CAD susceptibility and/or severity. TaqMan Real-Time genotyping for PUNISHER rs12318065A/C, SENCR rs12420823C/T, MIAT rs1061540C/T, MALAT1 rs3200401T/C, and GATA6-AS1 rs73390820A/G were run on the extracted genomic DNA from 100 unrelated patients with stable CAD undergoing diagnostic coronary angiography and from 100 controls. After adjusting covariates, the studied variants showed no association with disease susceptibility; however, MIAT*T/T genotype was associated with a more severe Gensini score. In contrast, MALAT1*T/C heterozygosity was associated with a lower score. The lipid profile, and to a lesser extent smoking status, male sex, weight, hypertension, and MALAT1 (T > C) (negative correlation), explained the variance between patients/control groups via a principal component analysis. Incorporating the principal components into a logistic regression model to predict CAD yielded a 0.92 AUC. In conclusion: MIAT rs1061540 and MALAT1 rs3200401 variants were associated with CAD severity and Gensini score in the present sample of the Egyptian population. Further large multi-center and functional analyses are needed to confirm the results and identify the underlying molecular mechanisms.

Citing Articles

Hypoxia triggers cardiomyocyte apoptosis via regulating the mA methylation-mediated LncMIAT/miR-708-5p/p53 axis.

Shen C, Chen X, Lin Y, Yang Y Heliyon. 2024; 10(11):e32455.

PMID: 38961902 PMC: 11219354. DOI: 10.1016/j.heliyon.2024.e32455.

Angio-Long Noncoding RNA MALAT1 (rs3200401) and MIAT (rs1061540) Gene Variants in Ovarian Cancer.

Fawzy M, Ibrahiem A, Osman D, Almars A, Alshammari M, Almazyad L Epigenomes. 2024; 8(1).

PMID: 38390896 PMC: 10885055. DOI: 10.3390/epigenomes8010005.

Long Non-coding RNA Genes Polymorphisms H19 (rs2251375) and MALAT1 (rs3200401) Association with Rheumatoid Arthritis and Their Correlation with Disease Activity in a Cohort of Egyptian Patients: A Pilot Study.

El-Sayed E, Fathy A, Al-Deen Younes S, Al-Shahaly M, Omar H Biochem Genet. 2023; 61(6):2443-2456.

PMID: 37103602 DOI: 10.1007/s10528-023-10383-z.

Migration/Differentiation-Associated LncRNA rs12420823*C/T: A Novel Gene Variant Can Predict Survival and Recurrence in Patients with Breast Cancer.

Al Ageeli E, Attallah S, Mohamed M, Almars A, Kattan S, Toraih E Genes (Basel). 2022; 13(11).

PMID: 36360233 PMC: 9690295. DOI: 10.3390/genes13111996.

Advances in Molecular Biomarkers in Cardiology.

Scicchitano P, Cameli M Biomolecules. 2022; 12(10).

PMID: 36291738 PMC: 9599388. DOI: 10.3390/biom12101530.

References

Sohrabifar N, Ghaderian S, Alipour Parsa S, Ghaedi H, Jafari H . Variation in the expression level of MALAT1, MIAT and XIST lncRNAs in coronary artery disease patients with and without type 2 diabetes mellitus. Arch Physiol Biochem. 2020; 128(5):1308-1315. DOI: 10.1080/13813455.2020.1768410. View

Garratt H, Ashburn R, Sopic M, Nogara A, Caporali A, Mitic T . Long Non-Coding RNA Regulation of Epigenetics in Vascular Cells. Noncoding RNA. 2021; 7(4). PMC: 8544676. DOI: 10.3390/ncrna7040062. View

Wang G, Li Y, Peng Y, Tang J, Li H . Association of polymorphisms in MALAT1 with risk of coronary atherosclerotic heart disease in a Chinese population. Lipids Health Dis. 2018; 17(1):75. PMC: 5891990. DOI: 10.1186/s12944-018-0728-2. View

Schussler J . Effectiveness and safety of transradial artery access for cardiac catheterization. Proc (Bayl Univ Med Cent). 2011; 24(3):205-9. PMC: 3124904. DOI: 10.1080/08998280.2011.11928716. View

Neumann P, Jae N, Knau A, Glaser S, Fouani Y, Rossbach O . The lncRNA GATA6-AS epigenetically regulates endothelial gene expression via interaction with LOXL2. Nat Commun. 2018; 9(1):237. PMC: 5770451. DOI: 10.1038/s41467-017-02431-1. View

Graffelman J, Jain D, Weir B . A genome-wide study of Hardy-Weinberg equilibrium with next generation sequence data. Hum Genet. 2017; 136(6):727-741. PMC: 5429372. DOI: 10.1007/s00439-017-1786-7. View

Bell R, Long X, Lin M, Bergmann J, Nanda V, Cowan S . Identification and initial functional characterization of a human vascular cell-enriched long noncoding RNA. Arterioscler Thromb Vasc Biol. 2014; 34(6):1249-59. PMC: 4024079. DOI: 10.1161/ATVBAHA.114.303240. View

Vausort M, Wagner D, Devaux Y . Long noncoding RNAs in patients with acute myocardial infarction. Circ Res. 2014; 115(7):668-77. DOI: 10.1161/CIRCRESAHA.115.303836. View

Said M, van de Vegte Y, Mobeen Zafar M, van der Ende M, Raja G, Verweij N . Contributions of Interactions Between Lifestyle and Genetics on Coronary Artery Disease Risk. Curr Cardiol Rep. 2019; 21(9):89. PMC: 6661028. DOI: 10.1007/s11886-019-1177-x. View

10.

Zhong X, Ma X, Zhang L, Li Y, Li Y, He R . MIAT promotes proliferation and hinders apoptosis by modulating miR-181b/STAT3 axis in ox-LDL-induced atherosclerosis cell models. Biomed Pharmacother. 2017; 97:1078-1085. DOI: 10.1016/j.biopha.2017.11.052. View

11.

Ozdemir B, Emul A, Ozdemir L, Sag S, Bicer M, Aydinlar A . Association of Aortic Diameters with Coronary Artery Disease Severity and Albumin Excretion. Biomed Res Int. 2015; 2015:857628. PMC: 4564612. DOI: 10.1155/2015/857628. View

12.

Li Y, Zhang D, Zhang Y, Xu X, Bi L, Zhang M . Association of lncRNA polymorphisms with triglyceride and total cholesterol levels among myocardial infarction patients in Chinese population. Gene. 2019; 724:143684. DOI: 10.1016/j.gene.2019.02.085. View

13.

Fawzy M, Toraih E, Aly N, Fakhr-Eldeen A, Badran D, Hussein M . Atherosclerotic and thrombotic genetic and environmental determinants in Egyptian coronary artery disease patients: a pilot study. BMC Cardiovasc Disord. 2017; 17(1):26. PMC: 5237236. DOI: 10.1186/s12872-016-0456-3. View

14.

Hu W, Ding H, Ouyang A, Zhang X, Xu Q, Han Y . LncRNA MALAT1 gene polymorphisms in coronary artery disease: a case-control study in a Chinese population. Biosci Rep. 2019; 39(3). PMC: 6422883. DOI: 10.1042/BSR20182213. View

15.

Yu B, Wang S . Angio-LncRs: LncRNAs that regulate angiogenesis and vascular disease. Theranostics. 2018; 8(13):3654-3675. PMC: 6037039. DOI: 10.7150/thno.26024. View

16.

Mohammad H, Abdelghany A, Al Ageeli E, Kattan S, Hassan R, Toraih E . Long Non-Coding RNAs Gene Variants as Molecular Markers for Diabetic Retinopathy Risk and Response to Anti-VEGF Therapy. Pharmgenomics Pers Med. 2021; 14:997-1014. PMC: 8374537. DOI: 10.2147/PGPM.S322463. View

17.

Azat M, Huojiahemaiti X, Gao R, Peng P . Long noncoding RNA MIAT: A potential role in the diagnosis and mediation of acute myocardial infarction. Mol Med Rep. 2019; 20(6):5216-5222. DOI: 10.3892/mmr.2019.10768. View

18.

Liao J, He Q, Li M, Chen Y, Liu Y, Wang J . LncRNA MIAT: Myocardial infarction associated and more. Gene. 2015; 578(2):158-61. DOI: 10.1016/j.gene.2015.12.032. View

19.

Lyu Q, Xu S, Lyu Y, Choi M, Christie C, Slivano O . stabilizes vascular endothelial cell adherens junctions through interaction with CKAP4. Proc Natl Acad Sci U S A. 2018; 116(2):546-555. PMC: 6329947. DOI: 10.1073/pnas.1810729116. View

20.

Zhu R, Liu X, He Z . Long non-coding RNA H19 and MALAT1 gene variants in patients with ischemic stroke in a northern Chinese Han population. Mol Brain. 2018; 11(1):58. PMC: 6180423. DOI: 10.1186/s13041-018-0402-7. View