Gene Expression Assessment in Advanced Mutant Rice Genotypes Under Drought Stress

Overview

Journal Int J Genomics

Publisher Wiley

Date 2020 Feb 22

PMID 32083115

Citations 11

Authors

Zarifth Shafika Kamarudin

Mohd Rafii Yusop

Mohd Razi Ismail

Mahmud Tengku Muda Mohamed

Abdul Rahim Harun

Oladosu Yusuff

Usman Magaji

Arolu Fatai

Affiliations

Soon will be listed here.

Abstract

Late embryogenesis abundant (LEA) proteins are primarily found in plants stem, roots, and other organs and play significant roles in tolerance to several abiotic stresses. Plants synthesize a discrete set of LEA proteins in response to drought stress. In this study, the expression patterns of genes were investigated in two advanced mutant rice genotypes subjected to the drought stress condition and different physiological traits including photosynthetic rate, leaf chlorophyll content, and photosystem II (PSII) photochemical efficiency () which were analyzed to confirm their drought tolerance. Five genes (, , , , and ) were used in the evaluation of rice genotypes and were significantly upregulated by more than 4-fold for MR219-4 and MR219-9. The upregulated genes by these two varieties showed high similarity with the drought-tolerant check variety, Aeron1. This indicates that these advanced mutant genotypes have better tolerance to drought stress. The changes in the expression level of genes among the selected rice genotypes under drought stress were further confirmed. Hence, genes could be served as a potential tool for drought tolerance determination in rice. MR219-4 and MR219-9 were found to be promising in breeding for drought tolerance as they offer better physiological adaptation to drought stress.

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