The N-glycosylation of Equine Tetherin Affects Antiviral Activity by Regulating Its Subcellular Localization

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2020 Feb 22

PMID 32079099

Citations 5

Authors

BoWen Bai

Xue-Feng Wang

Mengmeng Zhang

Lei Na

Xiangmin Zhang

Haili Zhang

Zhibiao Yang

Xiaojun Wang

Affiliations

Soon will be listed here.

Abstract

Tetherin is an interferon-inducible type II transmembrane glycoprotein which inhibits the release of viruses, including retroviruses, through a "physical tethering" model. However, the role that the glycosylation of tetherin plays in its antiviral activity remains controversial. In this study, we found that mutation of N-glycosylation sites resulted in an attenuation of the antiviral activity of equine tetherin (eqTHN), as well as a reduction in the expression of eqTHN at the plasma membrane (PM). In addition, eqTHN N-glycosylation mutants colocalize obviously with ER, CD63, LAMP1 and endosomes, while WT eqTHN do not. Furthermore, we also found that N-glycosylation impacts the transport of eqTHN in the cell not by affecting the endocytosis, but rather by influencing the anterograde trafficking of the protein. These results suggest that the N-glycosylation of eqTHN is important for the antiviral activity of the protein through regulating its normal subcellular localization. This finding will enhance our understanding of the function of this important restriction factor.

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