Insights into the Roles of Sperm in Animal Cloning

Overview

Journal Stem Cell Res Ther

Publisher Biomed Central

Date 2020 Feb 20

PMID 32070430

Citations 5

Authors

Pengxiang Qu

Yongsheng Wang

Chengsheng Zhang

Enqi Liu

Affiliations

Soon will be listed here.

Abstract

Somatic cell nuclear transfer (SCNT) has shown a wide application in the generation of transgenic animals, protection of endangered animals, and therapeutic cloning. However, the efficiency of SCNT remains very low due to some poorly characterized key factors. Compared with fertilized embryos, somatic donor cells lack some important components of sperm, such as sperm small noncoding RNA (sncRNA) and proteins. Loss of these factors is considered an important reason for the abnormal development of SCNT embryo. This study focused on recent advances of SCNT and the roles of sperm in development. Sperm-derived factors play an important role in nucleus reprogramming and cytoskeleton remodeling during SCNT embryo development. Hence, considering the role of sperm may provide a new strategy for improving cloning efficiency.

Citing Articles

Somatic Cell Nuclear Transfer in Rabbits.

Qu P, Cao W, Liu E Methods Mol Biol. 2023; 2647:169-181.

PMID: 37041334 DOI: 10.1007/978-1-0716-3064-8_8.

Loss of Renewal of Extracellular Vesicles: Harmful Effects on Embryo Development in vitro.

Qu P, Zhao J, Hu H, Cao W, Zhang Y, Qi J Int J Nanomedicine. 2022; 17:2301-2318.

PMID: 35615541 PMC: 9126234. DOI: 10.2147/IJN.S354003.

A New Toolbox in Experimental Embryology-Alternative Model Organisms for Studying Preimplantation Development.

Springer C, Wolf E, Simmet K J Dev Biol. 2021; 9(2).

PMID: 33918361 PMC: 8167745. DOI: 10.3390/jdb9020015.

Manipulating the Epigenome in Nuclear Transfer Cloning: Where, When and How.

Simmet K, Wolf E, Zakhartchenko V Int J Mol Sci. 2020; 22(1).

PMID: 33379395 PMC: 7794987. DOI: 10.3390/ijms22010236.

Double sperm cloning (DSC) is a promising strategy in mammalian genetic engineering and stem cell research.

Zhang Z, Zhang J, Huang S, He X, Deng L Stem Cell Res Ther. 2020; 11(1):388.

PMID: 32894201 PMC: 7487873. DOI: 10.1186/s13287-020-01907-0.

References

Pan G, Wang T, Yao H, Pei D . Somatic cell reprogramming for regenerative medicine: SCNT vs. iPS cells. Bioessays. 2012; 34(6):472-6. DOI: 10.1002/bies.201100174. View

Lesch B, Tothova Z, Morgan E, Liao Z, Bronson R, Ebert B . Intergenerational epigenetic inheritance of cancer susceptibility in mammals. Elife. 2019; 8. PMC: 6456297. DOI: 10.7554/eLife.39380. View

Penny G, Kay G, Sheardown S, Rastan S, Brockdorff N . Requirement for Xist in X chromosome inactivation. Nature. 1996; 379(6561):131-7. DOI: 10.1038/379131a0. View

Chesne P, Adenot P, Viglietta C, Baratte M, Boulanger L, Renard J . Cloned rabbits produced by nuclear transfer from adult somatic cells. Nat Biotechnol. 2002; 20(4):366-9. DOI: 10.1038/nbt0402-366. View

Selokar N, Sharma P, Saini M, Sheoran S, Rajendran R, Kumar D . Successful cloning of a superior buffalo bull. Sci Rep. 2019; 9(1):11366. PMC: 6684639. DOI: 10.1038/s41598-019-47909-8. View

Shi D, Lu F, Wei Y, Cui K, Yang S, Wei J . Buffalos (Bubalus bubalis) cloned by nuclear transfer of somatic cells. Biol Reprod. 2007; 77(2):285-91. DOI: 10.1095/biolreprod.107.060210. View

Baguisi A, Behboodi E, Melican D, Pollock J, Destrempes M, Cammuso C . Production of goats by somatic cell nuclear transfer. Nat Biotechnol. 1999; 17(5):456-61. DOI: 10.1038/8632. View

Matoba S, Wang H, Jiang L, Lu F, Iwabuchi K, Wu X . Loss of H3K27me3 Imprinting in Somatic Cell Nuclear Transfer Embryos Disrupts Post-Implantation Development. Cell Stem Cell. 2018; 23(3):343-354.e5. PMC: 6326833. DOI: 10.1016/j.stem.2018.06.008. View

Mastromonaco G, King W . Cloning in companion animal, non-domestic and endangered species: can the technology become a practical reality?. Reprod Fertil Dev. 2007; 19(6):748-61. DOI: 10.1071/rd07034. View

10.

Tucci V, Isles A, Kelsey G, Ferguson-Smith A . Genomic Imprinting and Physiological Processes in Mammals. Cell. 2019; 176(5):952-965. DOI: 10.1016/j.cell.2019.01.043. View

11.

Katayama M, Zhong Z, Lai L, Sutovsky P, Prather R, Schatten H . Mitochondrial distribution and microtubule organization in fertilized and cloned porcine embryos: implications for developmental potential. Dev Biol. 2006; 299(1):206-20. PMC: 1852431. DOI: 10.1016/j.ydbio.2006.07.022. View

12.

Gall L, Brochard V, Ruffini S, Laffont L, Fleurot R, Lavin T . Intermediate filaments promote nuclear mechanical constraints during somatic cell nuclear transfer in the mouse. Cell Reprogram. 2012; 14(6):497-504. DOI: 10.1089/cell.2012.0027. View

13.

Kim M, Jang G, Oh H, Yuda F, Kim H, Hwang W . Endangered wolves cloned from adult somatic cells. Cloning Stem Cells. 2007; 9(1):130-7. DOI: 10.1089/clo.2006.0034. View

14.

Simerly C, Navara C, Hyun S, Lee B, Kang S, Capuano S . Embryogenesis and blastocyst development after somatic cell nuclear transfer in nonhuman primates: overcoming defects caused by meiotic spindle extraction. Dev Biol. 2004; 276(2):237-52. DOI: 10.1016/j.ydbio.2004.10.006. View

15.

Zhao M, Chen H, Liu Q, Shao N, Sayed N, Wo H . Molecular and functional resemblance of differentiated cells derived from isogenic human iPSCs and SCNT-derived ESCs. Proc Natl Acad Sci U S A. 2017; 114(52):E11111-E11120. PMC: 5748177. DOI: 10.1073/pnas.1708991114. View

16.

Shin T, Kraemer D, Pryor J, Liu L, Rugila J, Howe L . A cat cloned by nuclear transplantation. Nature. 2002; 415(6874):859. DOI: 10.1038/nature723. View

17.

Lee B, Kim M, Jang G, Oh H, Yuda F, Kim H . Dogs cloned from adult somatic cells. Nature. 2005; 436(7051):641. DOI: 10.1038/436641a. View

18.

Loi P, Iuso D, Czernik M, Ogura A . A New, Dynamic Era for Somatic Cell Nuclear Transfer?. Trends Biotechnol. 2016; 34(10):791-797. DOI: 10.1016/j.tibtech.2016.03.008. View

19.

Mitalipov S, Zhou Q, Byrne J, Ji W, Norgren R, Wolf D . Reprogramming following somatic cell nuclear transfer in primates is dependent upon nuclear remodeling. Hum Reprod. 2007; 22(8):2232-42. DOI: 10.1093/humrep/dem136. View

20.

Zhang J, Qu P, Zhou C, Liu X, Ma X, Wang M . MicroRNA-125b is a key epigenetic regulatory factor that promotes nuclear transfer reprogramming. J Biol Chem. 2017; 292(38):15916-15926. PMC: 5612121. DOI: 10.1074/jbc.M117.796771. View