Comparison Between the SFTS-QS Kit and the PowerChek SFTSV Real-time PCR Kit for the Detection of Severe Fever With Thrombocytopenia Syndrome Virus

Overview

Journal Ann Lab Med

Specialty Pathology

Date 2020 Feb 19

PMID 32067431

Citations 2

Authors

In Young Yoo

Ji Youn Kim

Young Kyung Yoon

Hee Jae Huh

Nam Yong Lee

Affiliations

Soon will be listed here.

Abstract

The recent increase in severe fever with thrombocytopenia syndrome (SFTS) cases has led to the development of the SFTS-QS kit (MiCoBioMed, Seongnam, Korea) for detecting the SFTS virus (SFTSV, now renamed ). SFTS-QS is a qualitative real-time reverse transcription PCR assay based on lab-on-a-chip technology. We evaluated the performance of the SFTS-QS kit and compared it with that of the PowerChek SFTSV Real-time PCR kit (PowerChek; Kogene Biotech, Seoul, Korea). A total of 117 serum samples were simultaneously assayed using the SFTS-QS and PowerChek kits. Sanger sequencing targeting the S and M segments of SFTSV was performed as the reference method. The total turnaround time of the two kits was compared. The SFTS-QS results agreed with those of PowerChek with a kappa value of 0.92. The diagnostic sensitivity and specificity of the SFTS-QS kit were both 100% (14/14 and 103/103, respectively), whereas those of the PowerChek kit were 100% (14/14) and 98.1% (101/103), respectively. The results of SFTS-QS and PowerChek were comparable; however, the SFTS-QS kit required a shorter total turnaround time. The SFTS-QS kit produced accurate and fast results and thus could serve as a useful tool for detecting SFTSV.

Citing Articles

Accuracy of reverse-transcription polymerase chain reaction and loop-mediated isothermal amplification in diagnosing severe fever with thrombocytopenia syndrome: A systematic review and meta-analysis.

Tian W, Ren X, Gao X, Zhang Y, Chen Z, Zhang W J Med Virol. 2022; 94(12):5922-5932.

PMID: 35968756 PMC: 9804528. DOI: 10.1002/jmv.28068.

Developing a multiplex loop-mediated isothermal amplification assay (LAMP) to determine severe fever with thrombocytopenia syndrome (SFTS) and scrub typhus.

Jang W, Lim D, Choe Y, Nam J, Moon K, Kim C PLoS One. 2022; 17(2):e0262302.

PMID: 35171943 PMC: 8849512. DOI: 10.1371/journal.pone.0262302.

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