A Rapid and Simple Bead-bashing-based Method for Genomic DNA Extraction from Mammalian Tissue

Overview

Journal Biotechniques

Specialties Biomedical Engineering
Biotechnology

Date 2020 Feb 15

PMID 32054310

Citations 4

Authors

Shan Wei

Brynn Levy

Nataly Hoffman

Claudia Cujar

Reunet Rodney-Sandy

Ronald Wapner

Mary DAlton

Zev Williams

Affiliations

Soon will be listed here.

Abstract

Conventional genomic DNA (gDNA) extraction methods can take hours to complete, may require fume hoods and represent the most time-consuming step in many gDNA-based molecular assays. We systematically optimized a bead bashing-based (BBB) approach for rapid gDNA extraction without the need for a fume hood. Human tissue specimens (n = 34) subjected to the 12-min BBB method yielded 0.40 ± 0.17 (mean ± SD) μg of gDNA per milligram of tissue, sufficient for many downstream applications, and 3- and 6-min extensions resulted in an additional 0.43 ± 0.23 μg and 0.48 ± 0.43 μg per milligram of tissue, respectively. The BBB method provides a simple and rapid method for gDNA extraction from mammalian tissue that is applicable to time-sensitive clinical applications.

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