Development of a Novel Murine Model of Lymphatic Metastasis

Overview

Journal Clin Exp Metastasis

Specialty Oncology

Date 2020 Feb 14

PMID 32052231

Citations 4

Authors

Babak Banan

Jacob A Beckstead

Lauren E Dunavant

Yoojin Sohn

Jamie M Adcock

Sachiyo Nomura

Naji Abumrad

James R Goldenring

Barbara Fingleton

Affiliations

Soon will be listed here.

Abstract

Current laboratory models of lymphatic metastasis generally require either genetically modified animals or are technically challenging. Herein, we have developed a robust protocol for the induction of intralymphatic metastasis in wild-type mice with reproducible outcomes. To determine an optimal injection quantity and timeline for tumorigenesis, C57Bl/6 mice were injected directly into the mesenteric lymph duct (MLD) with varying numbers of syngeneic murine colon cancer cells (MC38) or gastric cancer cells (YTN16) expressing GFP/luciferase and monitored over 2-4 weeks. Tumor growth was tracked via whole-animal in vivo bioluminescence imaging (IVIS). Our data indicate that the injection of tumor cells into the MLD is a viable model for lymphatic metastasis as necropsies revealed large tumor burdens and metastasis in regional lymph nodes. This protocol enables a closer study of the role of lymphatics in cancer metastasis and opens a window for the development of novel approaches for treatment of metastatic diseases.

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