ATeam Technology for Detecting Early Signs of Viral Cytopathic Effect

Overview

Journal J Vet Med Sci

Specialty Veterinary Medicine

Date 2020 Feb 14

PMID 32051347

Authors

Karla Cristine C Doysabas

Mami Oba

Tomoki Ishibashi

Hideki Shibata

Hitoshi Takemae

Hiroshi Shimoda

Ronald Tarigan

Tetsuya Mizutani

Atsuo Iida

Eiichi Hondo

Affiliations

Soon will be listed here.

Abstract

Adenosine 5'-triphosphate (ATP), the major energy currency of the cell, is involved in many cellular processes, including the viral life cycle, and can be used as an indicator of early signs of cytopathic effect (CPE). In this study, we demonstrated that CPE can be analyzed using an FRET-based ATP probe named ATP indicator based on Epsilon subunit for Analytical Measurements (ATeam). The results revealed that as early as 3 hr, the virus infected cells showed a significantly different Venus/cyan fluorescent protein (CFP) ratio compared to the mock-infected cells. The ATeam technology is therefore useful to determine the early signs of ATP-based CPE as early as 3 hr without morphology-based CPE by light microscopy, and enables high throughput determination of the presence of microorganisms in neglected samples stored in laboratories.

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