UHPLC-ESI-Orbitrap-MS Analysis of Biologically Active Extracts from (Lour.) Merr. and L. Leaves

Overview

Journal Evid Based Complement Alternat Med

Specialty Rehabilitation Medicine

Date 2020 Feb 13

PMID 32047522

Citations 8

Authors

Machap Chandradevan

Sanimah Simoh

Ahmed Mediani

Nor Hadiani Ismail

Intan Safinar Ismail

Faridah Abas

Affiliations

Soon will be listed here.

Abstract

This study aimed to determine the total phenolic content, DPPH scavenging, -glucosidase, and nitric oxide (NO) inhibition of and extracts obtained with five different ethanolic concentrations. The findings showed that the 100% ethanolic extract of had the highest phenolic content and the lowest IC values for DPPH scavenging and NO inhibition activity compared to the properties of the other extracts. For , the 20% and 100% ethanolic extracts had comparably high total phenolic contents, and the latter possessed the lowest IC value in the NO inhibition assay. In addition, the 20% ethanolic extract of had the lowest IC value in the DPPH scavenging assay. However, none of the extracts from either herb had the ability to inhibit -glucosidase enzyme. Pearson correlation analysis indicated a strong relationship between the phenolic content and DPPH scavenging activity in both herb extracts. A moderately strong relationship was also observed between the phenolic content and NO inhibition in extracts and not in extracts. The UHPLC-ESI-Orbitrap-MS revealed major phenolics from the groups of hydroxycinnamic acids, hydroxybenzoic acids, and flavonoid derivatives from both herbs, which could be the key contributors to their bioactivities. Among the identified metabolites, 24 metabolites were tentatively assigned for the first time from both species of studied herbs. These two herbs could be recommended as prospective natural products with valuable medicinal properties.

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