Multidose Intramuscular Allogeneic Adipose Stem Cells Decrease the Severity of Canine Atopic Dermatitis: A Pilot Study

Overview

Journal Vet World

Specialty Veterinary Medicine

Date 2020 Feb 7

PMID 32025111

Citations 6

Authors

Nathaly Enciso

Jose Amiel

John Pando

Javier Enciso

Affiliations

Soon will be listed here.

Abstract

Aim: The aim of this pilot study was to evaluate the therapeutic and safety performance of an intramuscular treatment protocol of multidose of allogeneic adipose stem cells (ASCs) isolated, characterized, and expanded from a healthy canine donor.

Materials And Methods: Twelve dogs diagnosed with canine atopic dermatitis (CAD) were intramuscularly treated with 0.5×10 of cryopreserved ASCs from a healthy immunized young canine free donor weekly for 6 weeks. Treatment efficacy was evaluated by the pruritus index and the CAD Lesion Index (CADLI) test. Safety and adverse effects were determined by injection site reaction, weight, blood chemistry, liver function, and whole blood count.

Results: Canine ASCs obtained from a donor met the minimum qualities required for this type of cells and showed viability of 90% after thawing. The efficacy of the CADLI score and the pruritus index in 12 dogs with atopic dermatitis was statistically significant efficacy. No adverse reactions were observed at the intramuscular application site, or in relation to animal weight, blood cell populations, or liver and renal function.

Conclusion: These results suggest that intramuscular administration of cryopreserved ASCs to dogs with atopic dermatitis is a promising cellular therapeutic product for the relief of the symptoms of this disease; however, the duration of the effects obtained with this dose and with other doses should be evaluated, as well as possible immune reactions. As far as we know, this is the first report of the use of multiple intramuscular doses cryopreserved ASCs to treat atopic dermatitis.

Citing Articles

In Situ Treatment of Refractory Perianal Fistulas in Dogs with Low-Dose Allogeneic Adipose-Derived Mesenchymal Stem Cells.

Enciso N, Enciso-Benavides J, Sandoval J, Enciso J Animals (Basel). 2024; 14(22).

PMID: 39595352 PMC: 11591124. DOI: 10.3390/ani14223300.

Current Advances in Mesenchymal Stem Cell Therapies Applied to Wounds and Skin, Eye, and Neuromuscular Diseases in Companion Animals.

Picazo R, Rojo C, Rodriguez-Quiros J, Gonzalez-Gil A Animals (Basel). 2024; 14(9).

PMID: 38731367 PMC: 11083242. DOI: 10.3390/ani14091363.

The Stromal Vascular Fraction from Canine Adipose Tissue Contains Mesenchymal Stromal Cell Subpopulations That Show Time-Dependent Adhesion to Cell Culture Plastic Vessels.

Scattini G, Pellegrini M, Severi G, Cagiola M, Pascucci L Animals (Basel). 2023; 13(7).

PMID: 37048431 PMC: 10093060. DOI: 10.3390/ani13071175.

The effect of uterine-derived mesenchymal stromal cells for the treatment of canine atopic dermatitis: A pilot study.

Black L, Zacharias S, Hughes M, Bautista R, Taechangam N, Sand T Front Vet Sci. 2022; 9:1011174.

PMID: 36213399 PMC: 9538998. DOI: 10.3389/fvets.2022.1011174.

First report of canine morbillivirus infection of adipose tissue-derived stem cells from dogs with distemper.

Altamirano-Samaniego F, Enciso-Benavides J, Rojas N, Manuel Iglesias-Pedraz J, Enciso N, Fossatti M Vet World. 2022; 15(7):1835-1842.

PMID: 36185532 PMC: 9394121. DOI: 10.14202/vetworld.2022.1835-1842.

References

Ra J, Jeong E, Kang S, Lee S, Choi K . A Prospective, Nonrandomized, no Placebo-Controlled, Phase I/II Clinical Trial Assessing the Safety and Efficacy of Intramuscular Injection of Autologous Adipose Tissue-Derived Mesenchymal Stem Cells in Patients With Severe Buerger's Disease. Cell Med. 2017; 9(3):87-102. PMC: 5509019. DOI: 10.3727/215517916X693069. View

Gonzales A, Fleck T, Humphrey W, Galvan B, Aleo M, Mahabir S . IL-31-induced pruritus in dogs: a novel experimental model to evaluate anti-pruritic effects of canine therapeutics. Vet Dermatol. 2015; 27(1):34-e10. PMC: 4737310. DOI: 10.1111/vde.12280. View

Popa I, Remoue N, Osta B, Pin D, Gatto H, Haftek M . The lipid alterations in the stratum corneum of dogs with atopic dermatitis are alleviated by topical application of a sphingolipid-containing emulsion. Clin Exp Dermatol. 2012; 37(6):665-71. DOI: 10.1111/j.1365-2230.2011.04313.x. View

Bunnell B, Flaat M, Gagliardi C, Patel B, Ripoll C . Adipose-derived stem cells: isolation, expansion and differentiation. Methods. 2008; 45(2):115-20. PMC: 3668445. DOI: 10.1016/j.ymeth.2008.03.006. View

Plant J, Neradilek M . Effectiveness of regionally-specific immunotherapy for the management of canine atopic dermatitis. BMC Vet Res. 2017; 13(1):4. PMC: 5216569. DOI: 10.1186/s12917-016-0917-z. View

Zhang Y, Meng Q, Zhang Y, Chen X, Wang Y . Adipose-derived mesenchymal stem cells suppress of acute rejection in small bowel transplantation. Saudi J Gastroenterol. 2017; 23(6):323-329. PMC: 5738793. DOI: 10.4103/sjg.SJG_122_17. View

Dip R, Carmichael J, Letellier I, Strehlau G, Roberts E, Bensignor E . Concurrent short-term use of prednisolone with cyclosporine A accelerates pruritus reduction and improvement in clinical scoring in dogs with atopic dermatitis. BMC Vet Res. 2013; 9:173. PMC: 3847128. DOI: 10.1186/1746-6148-9-173. View

Olivry T, Banovic F . Treatment of canine atopic dermatitis: time to revise our strategy?. Vet Dermatol. 2019; 30(2):87-90. DOI: 10.1111/vde.12740. View

Petchdee S, Sompeewong S . Intravenous administration of puppy deciduous teeth stem cells in degenerative valve disease. Vet World. 2017; 9(12):1429-1434. PMC: 5234058. DOI: 10.14202/vetworld.2016.1429-1434. View

10.

Jee M, Im Y, Choi J, Kang S . Compensation of cATSCs-derived TGFβ1 and IL10 expressions was effectively modulated atopic dermatitis. Cell Death Dis. 2013; 4:e497. PMC: 3734835. DOI: 10.1038/cddis.2013.4. View

11.

Orciani M, Campanati A, Caffarini M, Ganzetti G, Consales V, Lucarini G . T helper (Th)1, Th17 and Th2 imbalance in mesenchymal stem cells of adult patients with atopic dermatitis: at the origin of the problem. Br J Dermatol. 2016; 176(6):1569-1576. DOI: 10.1111/bjd.15078. View

12.

Ribeiro A, Laranjeira P, Mendes S, Velada I, Leite C, Andrade P . Mesenchymal stem cells from umbilical cord matrix, adipose tissue and bone marrow exhibit different capability to suppress peripheral blood B, natural killer and T cells. Stem Cell Res Ther. 2014; 4(5):125. PMC: 3854702. DOI: 10.1186/scrt336. View

13.

Ivanova-Todorova E, Bochev I, Mourdjeva M, Dimitrov R, Bukarev D, Kyurkchiev S . Adipose tissue-derived mesenchymal stem cells are more potent suppressors of dendritic cells differentiation compared to bone marrow-derived mesenchymal stem cells. Immunol Lett. 2009; 126(1-2):37-42. DOI: 10.1016/j.imlet.2009.07.010. View

14.

Hill P, Lau P, Rybnicek J . Development of an owner-assessed scale to measure the severity of pruritus in dogs. Vet Dermatol. 2007; 18(5):301-8. DOI: 10.1111/j.1365-3164.2007.00616.x. View

15.

Plant J, Gortel K, Kovalik M, Polissar N, Neradilek M . Development and validation of the Canine Atopic Dermatitis Lesion Index, a scale for the rapid scoring of lesion severity in canine atopic dermatitis. Vet Dermatol. 2012; 23(6):515-e103. DOI: 10.1111/j.1365-3164.2012.01113.x. View

16.

Braid L, Wood C, Wiese D, Ford B . Intramuscular administration potentiates extended dwell time of mesenchymal stromal cells compared to other routes. Cytotherapy. 2017; 20(2):232-244. DOI: 10.1016/j.jcyt.2017.09.013. View

17.

Favrot C, Steffan J, Seewald W, Picco F . A prospective study on the clinical features of chronic canine atopic dermatitis and its diagnosis. Vet Dermatol. 2010; 21(1):23-31. DOI: 10.1111/j.1365-3164.2009.00758.x. View

18.

Hall M, Rosenkrantz W, Hong J, Griffin C, Mendelsohn C . Evaluation of the potential use of adipose-derived mesenchymal stromal cells in the treatment of canine atopic dermatitis: a pilot study. Vet Ther. 2010; 11(2):E1-14. View

19.

Blaskovic M, Rosenkrantz W, Neuber A, Sauter-Louis C, Mueller R . The effect of a spot-on formulation containing polyunsaturated fatty acids and essential oils on dogs with atopic dermatitis. Vet J. 2013; 199(1):39-43. DOI: 10.1016/j.tvjl.2013.10.024. View

20.

Bahamondes F, Flores E, Cattaneo G, Bruna F, Conget P . Omental adipose tissue is a more suitable source of canine Mesenchymal stem cells. BMC Vet Res. 2017; 13(1):166. PMC: 5465460. DOI: 10.1186/s12917-017-1053-0. View