Omental Adipose Tissue is a More Suitable Source of Canine Mesenchymal Stem Cells

Overview

Journal BMC Vet Res

Publisher Biomed Central

Specialty Veterinary Medicine

Date 2017 Jun 10

PMID 28595579

Citations 21

Authors

Francisca Bahamondes

Estefania Flores

Gino Cattaneo

Flavia Bruna

Paulette Conget

Affiliations

Soon will be listed here.

Abstract

Background: Mesenchymal Stem Cells (MSCs) are a promising therapeutic tool in veterinary medicine. Currently the subcutaneous adipose tissue is the leading source of MSCs in dogs. MSCs derived from distinct fat depots have shown dissimilarities in their accessibility and therapeutic potential. The aims of our work were to determine the suitability of omental adipose tissue as a source of MSCs, according to sampling success, cell yield and paracrine properties of isolated cells, and compared to subcutaneous adipose tissue.

Results: While sampling success of omental adipose tissue was 100% (14 collections from14 donors) for subcutaneous adipose tissue it was 71% (10 collections from 14 donors). MSCs could be isolated from both sources. Cell yield was significantly higher for omental than for subcutaneous adipose tissue (38 ± 1 vs. 30 ± 1 CFU-F/g tissue, p < 0.0001). No differences were observed between sources regarding cell proliferation potential (73 ± 1 vs. 74 ± 1 CDPL) and cell senescence (at passage 10, both cultures presented enlarged cells with cytoplasmic vacuoles and cellular debris). Omental- and subcutaneous-derived MSCs expressed at the same level bFGF, PDGF, HGF, VEGF, ANG1 and IL-10. Irrespective of the source, isolated MSCs induced proliferation, migration and vascularization of target cells, and inhibited the activation of T lymphocytes.

Conclusion: Compared to subcutaneous adipose tissue, omental adipose tissue is a more suitable source of MSCs in dogs. Since it can be procured from donors with any body condition, its collection procedure is always feasible, its cell yield is high and the MSCs isolated from it have desirable differentiation and paracrine potentials.

Citing Articles

Evaluation of a Novel Mechanical Device for the Production of Microfragmented Adipose Tissue for Veterinary Regenerative Medicine: A Proof-of-Concept.

Berni P, Andreoli V, Conti V, Ramoni R, Basini G, Scattini G Int J Mol Sci. 2024; 25(21).

PMID: 39519405 PMC: 11546731. DOI: 10.3390/ijms252111854.

Efficacy of a Single Injection of Stromal Vascular Fraction in Dogs with Elbow Osteoarthritis: A Clinical Prospective Study.

Bruns Y, Schroers M, Steigmeier-Raith S, Waselau A, Reese S, Meyer-Lindenberg A Animals (Basel). 2024; 14(19).

PMID: 39409752 PMC: 11475770. DOI: 10.3390/ani14192803.

The Omentum-A Forgotten Structure in Veterinary Surgery in Small Animals' Surgery.

Morawska-Kozlowska M, Wilkosz A, Zhalniarovich Y Animals (Basel). 2024; 14(13).

PMID: 38997960 PMC: 11240631. DOI: 10.3390/ani14131848.

Influence of the Anatomical Site on Adipose Tissue-Derived Stromal Cells' Biological Profile and Osteogenic Potential in Companion Animals.

Ferreira-Baptista C, Ferreira R, Fernandes M, Gomes P, Colaco B Vet Sci. 2023; 10(12).

PMID: 38133224 PMC: 10747344. DOI: 10.3390/vetsci10120673.

＜Editors' Choice＞ Supernatant from activated omentum accelerates wound healing in diabetic mice wound model.

Li Y, Hashikawa K, Ebisawa K, Kambe M, Higuchi S, Kamei Y Nagoya J Med Sci. 2023; 85(3):528-541.

PMID: 37829482 PMC: 10565575. DOI: 10.18999/nagjms.85.3.528.

References

Walker T, Moore S, Lawson M, Panettieri Jr R, Chilvers E . Platelet-derived growth factor-BB and thrombin activate phosphoinositide 3-kinase and protein kinase B: role in mediating airway smooth muscle proliferation. Mol Pharmacol. 1998; 54(6):1007-15. DOI: 10.1124/mol.54.6.1007. View

Reich C, Raabe O, Wenisch S, Bridger P, Kramer M, Arnhold S . Isolation, culture and chondrogenic differentiation of canine adipose tissue- and bone marrow-derived mesenchymal stem cells--a comparative study. Vet Res Commun. 2012; 36(2):139-48. DOI: 10.1007/s11259-012-9523-0. View

Vieira N, Brandalise V, Zucconi E, Secco M, Strauss B, Zatz M . Isolation, characterization, and differentiation potential of canine adipose-derived stem cells. Cell Transplant. 2009; 19(3):279-89. DOI: 10.3727/096368909X481764. View

ZuK P, Zhu M, Ashjian P, De Ugarte D, Huang J, Mizuno H . Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 2002; 13(12):4279-95. PMC: 138633. DOI: 10.1091/mbc.e02-02-0105. View

Ren G, Su J, Zhang L, Zhao X, Ling W, Lhuillie A . Species variation in the mechanisms of mesenchymal stem cell-mediated immunosuppression. Stem Cells. 2009; 27(8):1954-62. DOI: 10.1002/stem.118. View

Guercio A, Di Bella S, Casella S, Di Marco P, Russo C, Piccione G . Canine mesenchymal stem cells (MSCs): characterization in relation to donor age and adipose tissue-harvesting site. Cell Biol Int. 2013; 37(8):789-98. DOI: 10.1002/cbin.10090. View

Ezquer F, Ezquer M, Simon V, Conget P . The antidiabetic effect of MSCs is not impaired by insulin prophylaxis and is not improved by a second dose of cells. PLoS One. 2011; 6(1):e16566. PMC: 3029393. DOI: 10.1371/journal.pone.0016566. View

Lubinus M, Meier K, Smith E, Gause K, LeRoy E, Trojanowska M . Independent effects of platelet-derived growth factor isoforms on mitogen-activated protein kinase activation and mitogenesis in human dermal fibroblasts. J Biol Chem. 1994; 269(13):9822-5. View

Caplan A, Dennis J . Mesenchymal stem cells as trophic mediators. J Cell Biochem. 2006; 98(5):1076-84. DOI: 10.1002/jcb.20886. View

10.

Munn D, Zhou M, Attwood J, Bondarev I, Conway S, Marshall B . Prevention of allogeneic fetal rejection by tryptophan catabolism. Science. 1998; 281(5380):1191-3. DOI: 10.1126/science.281.5380.1191. View

11.

Haynesworth S, Baber M, Caplan A . Cytokine expression by human marrow-derived mesenchymal progenitor cells in vitro: effects of dexamethasone and IL-1 alpha. J Cell Physiol. 1996; 166(3):585-92. DOI: 10.1002/(SICI)1097-4652(199603)166:3<585::AID-JCP13>3.0.CO;2-6. View

12.

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

13.

Antoniades H, Scher C, Stiles C . Purification of human platelet-derived growth factor. Proc Natl Acad Sci U S A. 1979; 76(4):1809-13. PMC: 383481. DOI: 10.1073/pnas.76.4.1809. View

14.

Schuldiner M, Yanuka O, Itskovitz-Eldor J, Melton D, Benvenisty N . Effects of eight growth factors on the differentiation of cells derived from human embryonic stem cells. Proc Natl Acad Sci U S A. 2000; 97(21):11307-12. PMC: 17196. DOI: 10.1073/pnas.97.21.11307. View

15.

Strem B, Hicok K, Zhu M, Wulur I, Alfonso Z, Schreiber R . Multipotential differentiation of adipose tissue-derived stem cells. Keio J Med. 2005; 54(3):132-41. DOI: 10.2302/kjm.54.132. View

16.

Arnold F, West D . Angiogenesis in wound healing. Pharmacol Ther. 1991; 52(3):407-22. DOI: 10.1016/0163-7258(91)90034-j. View

17.

Russo V, Yu C, Belliveau P, Hamilton A, Flynn L . Comparison of human adipose-derived stem cells isolated from subcutaneous, omental, and intrathoracic adipose tissue depots for regenerative applications. Stem Cells Transl Med. 2013; 3(2):206-17. PMC: 3925056. DOI: 10.5966/sctm.2013-0125. View

18.

Wagner W, Horn P, Castoldi M, Diehlmann A, Bork S, Saffrich R . Replicative senescence of mesenchymal stem cells: a continuous and organized process. PLoS One. 2008; 3(5):e2213. PMC: 2374903. DOI: 10.1371/journal.pone.0002213. View

19.

Astor D, Hoelzler M, Harman R, Bastian R . Patient factors influencing the concentration of stromal vascular fraction (SVF) for adipose-derived stromal cell (ASC) therapy in dogs. Can J Vet Res. 2013; 77(3):177-82. PMC: 3700442. View

20.

Le Blanc K, Tammik L, Sundberg B, Haynesworth S, Ringden O . Mesenchymal stem cells inhibit and stimulate mixed lymphocyte cultures and mitogenic responses independently of the major histocompatibility complex. Scand J Immunol. 2003; 57(1):11-20. DOI: 10.1046/j.1365-3083.2003.01176.x. View