Diagnostic Testing for Crimean-Congo Hemorrhagic Fever

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2020 Feb 7

PMID 32024724

Citations 17

Authors

Vanessa N Raabe

Affiliations

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Abstract

Crimean-Congo hemorrhagic fever is the most geographically widespread tick-borne virus, with infection resulting in mortality in up to 30% of cases. Clinical diagnosis alone is difficult due to the nonspecific nature of symptoms; therefore, laboratory diagnostics should be utilized for patients with residence in or travel to regions of endemicity in whom the disease is suspected. This minireview provides an overview of laboratory tests available for Crimean-Congo hemorrhagic fever (CCHF) and their utility in diagnosis with a focus on diagnosing CCHF in humans.

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References

Shepherd A, Swanepoel R, Leman P . Antibody response in Crimean-Congo hemorrhagic fever. Rev Infect Dis. 1989; 11 Suppl 4:S801-6. DOI: 10.1093/clinids/11.supplement_4.s801. View

Appannanavar S, Mishra B . An update on crimean congo hemorrhagic Fever. J Glob Infect Dis. 2011; 3(3):285-92. PMC: 3162818. DOI: 10.4103/0974-777X.83537. View

Osman H, Eltom K, Musa N, Bilal N, Elbashir M, Aradaib I . Development and evaluation of loop-mediated isothermal amplification assay for detection of Crimean Congo hemorrhagic fever virus in Sudan. J Virol Methods. 2013; 190(1-2):4-10. DOI: 10.1016/j.jviromet.2013.03.004. View

Zahraei B, Hashemzadeh M, Najarasl M, Zahiriyeganeh S, Tat M, Metanat M . Novel, In-House, SYBR Green Based One-Step rRT-PCR: Rapid and Accurate Diagnosis of Crimean-Congo Hemorrhagic Fever Virus in Suspected Patients From Iran. Jundishapur J Microbiol. 2016; 9(1):e29246. PMC: 4834134. DOI: 10.5812/jjm.29246. View

Johnson S, Henschke N, Maayan N, Mills I, Buckley B, Kakourou A . Ribavirin for treating Crimean Congo haemorrhagic fever. Cochrane Database Syst Rev. 2018; 6:CD012713. PMC: 5994605. DOI: 10.1002/14651858.CD012713.pub2. View

Brinkmann A, Ergunay K, Radonic A, Tufan Z, Domingo C, Nitsche A . Development and preliminary evaluation of a multiplexed amplification and next generation sequencing method for viral hemorrhagic fever diagnostics. PLoS Negl Trop Dis. 2017; 11(11):e0006075. PMC: 5714388. DOI: 10.1371/journal.pntd.0006075. View

Rangunwala A, Samudzi R, Burt F . Detection of IgG antibody against Crimean-Congo haemorrhagic fever virus using ELISA with recombinant nucleoprotein antigens from genetically diverse strains. Epidemiol Infect. 2013; 142(10):2147-54. PMC: 9151302. DOI: 10.1017/S0950268813002987. View

Baniasadi V, Pouriayevali M, Jalali T, Fazlalipour M, Azadmanesh K, Salehi-Vaziri M . Evaluation of first rapid diagnostic kit for Anti-Crimean-Congo Hemorrhagic Fever virus IgM antibody using clinical samples from Iran. J Virol Methods. 2018; 265:49-52. DOI: 10.1016/j.jviromet.2018.12.015. View

Nasirian H . Crimean-Congo hemorrhagic fever (CCHF) seroprevalence: A systematic review and meta-analysis. Acta Trop. 2019; 196:102-120. DOI: 10.1016/j.actatropica.2019.05.019. View

10.

Cevik M, Erbay A, Bodur H, Eren S, Akinci E, Sener K . Viral load as a predictor of outcome in Crimean-Congo hemorrhagic fever. Clin Infect Dis. 2007; 45(7):e96-100. DOI: 10.1086/521244. View

11.

Garrison A, Alakbarova S, Kulesh D, Shezmukhamedova D, Khodjaev S, Endy T . Development of a TaqMan minor groove binding protein assay for the detection and quantification of Crimean-Congo hemorrhagic fever virus. Am J Trop Med Hyg. 2007; 77(3):514-20. View

12.

Dowall S, Richards K, Graham V, Chamberlain J, Hewson R . Development of an indirect ELISA method for the parallel measurement of IgG and IgM antibodies against Crimean-Congo haemorrhagic fever (CCHF) virus using recombinant nucleoprotein as antigen. J Virol Methods. 2011; 179(2):335-41. DOI: 10.1016/j.jviromet.2011.11.020. View

13.

Yapar M, Aydogan H, Pahsa A, Besirbellioglu B, Bodur H, Basustaoglu A . Rapid and quantitative detection of Crimean-Congo hemorrhagic fever virus by one-step real-time reverse transcriptase-PCR. Jpn J Infect Dis. 2005; 58(6):358-62. View

14.

Fisgin N, Doganci L, Tanyel E, Tulek N . Initial high rate of misdiagnosis in Crimean Congo haemorrhagic fever patients in an endemic region of Turkey. Epidemiol Infect. 2009; 138(1):139-44. DOI: 10.1017/S0950268809990318. View

15.

Leblebicioglu H, Ozaras R, Fletcher T, Beeching N . Crimean-Congo haemorrhagic fever in travellers: A systematic review. Travel Med Infect Dis. 2016; 14(2):73-80. PMC: 7110636. DOI: 10.1016/j.tmaid.2016.03.002. View

16.

Swanepoel R, Gill D, Shepherd A, Leman P, Mynhardt J, Harvey S . The clinical pathology of Crimean-Congo hemorrhagic fever. Rev Infect Dis. 1989; 11 Suppl 4:S794-800. DOI: 10.1093/clinids/11.supplement_4.s794. View

17.

Bonney L, Watson R, Afrough B, Mullojonova M, Dzhuraeva V, Tishkova F . A recombinase polymerase amplification assay for rapid detection of Crimean-Congo Haemorrhagic fever Virus infection. PLoS Negl Trop Dis. 2017; 11(10):e0006013. PMC: 5656326. DOI: 10.1371/journal.pntd.0006013. View

18.

Garrison A, Smith D, Golden J . Animal Models for Crimean-Congo Hemorrhagic Fever Human Disease. Viruses. 2019; 11(7). PMC: 6669593. DOI: 10.3390/v11070590. View

19.

Kamboj A, Pateriya A, Mishra A, Ranaware P, Kulkarni D, Raut A . Novel molecular beacon probe-based real-time RT-PCR assay for diagnosis of Crimean-Congo hemorrhagic fever encountered in India. Biomed Res Int. 2014; 2014:496219. PMC: 4022296. DOI: 10.1155/2014/496219. View

20.

Gaidamovich S, Klisenko G, Shanoyan N, Obukhova V, Melnikova E . Indirect hemagglutination for diagnosis of Crimean hemorrhagic fever. Intervirology. 1973; 2(3):181-5. DOI: 10.1159/000149421. View