Vitamin D/VDR Signaling Induces MiR-27a/b Expression in Oral Lichen Planus

Overview

Journal Sci Rep

Specialty Science

Date 2020 Jan 17

PMID 31942011

Citations 14

Authors

Xuejun Ge

Lu Yuan

Jizhen Wei

Tivoli Nguyen

Chenwei Tang

Wang Liao

Ran Li

Fang Yang

Fang Zhang

Bin Zhao

Jie Du

Affiliations

Soon will be listed here.

Abstract

MicroRNA-27a/b are small non-coding RNAs which are reported to regulate inflammatory response and cell proliferation. Although some studies have demonstrated that miR-27b is down-regulated in the oral specimens of patients suffering with oral lichen planus (OLP), the molecular mechanism of miR-27b decrease remains a large mystery, and the expression of miR-27a in OLP is not well explored. Here, we demonstrated both miR-27a and miR-27b, compared with healthy controls, were reduced in the oral biopsies, serum and saliva samples derived from OLP patients. The reductions of miR-27a/b were also confirmed in the lipopolysaccharide (LPS)- or activated CD4 T cell-treated human oral keratinocytes (HOKs). Furthermore, we found vitamin D receptor (VDR) binding sites in the promoters of miR-27a/b genes and verified this finding. We also tested miR-27a/b levels in the oral epithelium from paricalcitol-treated, vitamin D deficient or VDR knockout mice. In the rescue experiments, we confirmed vitamin D and VDR inhibited LPS- or activated CD4 T cell-induced miR-27a/b reductions in HOKs. In sum, our results show that vitamin D/VDR signaling induces miR-27a/b in oral lichen planus.

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References

van der Waal I . Potentially malignant disorders of the oral and oropharyngeal mucosa; terminology, classification and present concepts of management. Oral Oncol. 2008; 45(4-5):317-23. DOI: 10.1016/j.oraloncology.2008.05.016. View

Zhang W, Liu W, Zhou Y, Shen X, Wang Y, Tang G . Altered microRNA expression profile with miR-27b down-regulation correlated with disease activity of oral lichen planus. Oral Dis. 2011; 18(3):265-70. DOI: 10.1111/j.1601-0825.2011.01869.x. View

Cheng Y, Gould A, Kurago Z, Fantasia J, Muller S . Diagnosis of oral lichen planus: a position paper of the American Academy of Oral and Maxillofacial Pathology. Oral Surg Oral Med Oral Pathol Oral Radiol. 2016; 122(3):332-54. DOI: 10.1016/j.oooo.2016.05.004. View

Gorouhi F, Davari P, Fazel N . Cutaneous and mucosal lichen planus: a comprehensive review of clinical subtypes, risk factors, diagnosis, and prognosis. ScientificWorldJournal. 2014; 2014:742826. PMC: 3929580. DOI: 10.1155/2014/742826. View

Mendell J, Olson E . MicroRNAs in stress signaling and human disease. Cell. 2012; 148(6):1172-87. PMC: 3308137. DOI: 10.1016/j.cell.2012.02.005. View

Dumortier O, Hinault C, Van Obberghen E . MicroRNAs and metabolism crosstalk in energy homeostasis. Cell Metab. 2013; 18(3):312-24. DOI: 10.1016/j.cmet.2013.06.004. View

Vacchio M, Bosselut R . T cell metabolism: microRNAs cap PTEN to feed the expanding crowd. Immunity. 2013; 38(5):847-8. DOI: 10.1016/j.immuni.2013.05.006. View

Tao G, Martin J . MicroRNAs get to the heart of development. Elife. 2013; 2:e01710. PMC: 3833033. DOI: 10.7554/eLife.01710. View

Schuldt A . MicroRNAs diversify in Drosophila development. Nat Cell Biol. 2005; 7(8):781. DOI: 10.1038/ncb0805-781. View

10.

Pua H, Steiner D, Patel S, Gonzalez J, Ortiz-Carpena J, Kageyama R . MicroRNAs 24 and 27 Suppress Allergic Inflammation and Target a Network of Regulators of T Helper 2 Cell-Associated Cytokine Production. Immunity. 2016; 44(4):821-32. PMC: 4838571. DOI: 10.1016/j.immuni.2016.01.003. View

11.

Aghbari S, Gaafar S, Shaker O, Ashiry S, Zayed S . Evaluating the accuracy of microRNA27b and microRNA137 as biomarkers of activity and potential malignant transformation in oral lichen planus patients. Arch Dermatol Res. 2018; 310(3):209-220. DOI: 10.1007/s00403-018-1805-0. View

12.

Ahmadi-Motamayel F, Bayat Z, Hajilooi M, Shahryar-Hesami S, Mahdavinezhad A, Samie L . Evaluation of the miRNA-146a and miRNA-155 Expression Levels in Patients with Oral Lichen Planus. Iran J Immunol. 2017; 14(4):316-324. DOI: IJIv14i4A6. View

13.

Guo Y, Riley K, Iwasaki A, Steitz J . Alternative capture of noncoding RNAs or protein-coding genes by herpesviruses to alter host T cell function. Mol Cell. 2014; 54(1):67-79. PMC: 4039351. DOI: 10.1016/j.molcel.2014.03.025. View

14.

Ye P, Ke X, Zang X, Sun H, Dong Z, Lin J . Up-regulated MiR-27-3p promotes the G1-S phase transition by targeting inhibitor of growth family member 5 in osteosarcoma. Biomed Pharmacother. 2018; 101:219-227. DOI: 10.1016/j.biopha.2018.02.066. View

15.

Cruz L, Hashemifar S, Wu C, Cho S, Nguyen D, Lin L . Excessive expression of miR-27 impairs Treg-mediated immunological tolerance. J Clin Invest. 2017; 127(2):530-542. PMC: 5272185. DOI: 10.1172/JCI88415. View

16.

Bouillon R, Carmeliet G, Verlinden L, van Etten E, Verstuyf A, Luderer H . Vitamin D and human health: lessons from vitamin D receptor null mice. Endocr Rev. 2008; 29(6):726-76. PMC: 2583388. DOI: 10.1210/er.2008-0004. View

17.

Haussler M, Whitfield G, Haussler C, Hsieh J, Thompson P, Selznick S . The nuclear vitamin D receptor: biological and molecular regulatory properties revealed. J Bone Miner Res. 1998; 13(3):325-49. DOI: 10.1359/jbmr.1998.13.3.325. View

18.

Liu W, Chen Y, Golan M, Annunziata M, Du J, Dougherty U . Intestinal epithelial vitamin D receptor signaling inhibits experimental colitis. J Clin Invest. 2013; 123(9):3983-96. PMC: 3754241. DOI: 10.1172/JCI65842. View

19.

Zhao B, Li R, Yang F, Yu F, Xu N, Zhang F . LPS-induced Vitamin D Receptor Decrease in Oral Keratinocytes Is Associated With Oral Lichen Planus. Sci Rep. 2018; 8(1):763. PMC: 5768778. DOI: 10.1038/s41598-018-19234-z. View

20.

Ge X, Wang L, Li M, Xu N, Yu F, Yang F . Vitamin D/VDR signaling inhibits LPS-induced IFNγ and IL-1β in Oral epithelia by regulating hypoxia-inducible factor-1α signaling pathway. Cell Commun Signal. 2019; 17(1):18. PMC: 6391768. DOI: 10.1186/s12964-019-0331-9. View