Resistance Status of Anopheles Gambiae S.l. to Insecticides Following the 2011 Mass Distribution Campaign of Long-lasting Insecticidal Nets (LLINs) in the Plateau Department, South-eastern Benin

Overview

Journal Malar J

Publisher Biomed Central

Specialty Tropical Medicine

Date 2020 Jan 17

PMID 31941494

Citations 6

Authors

Arthur Sovi

Renaud Govoetchan

Razaki Osse

Come Z Koukpo

Albert S Salako

Thomas Syme

Rodrigue Anagonou

Augustin Fongnikin

Udoka C Nwangwu

Frederic Oke-Agbo

Filemon Tokponnon

Gil Germain Padonou

Martin Codjo Akogbeto

Affiliations

Soon will be listed here.

Abstract

Background: In 2011, Benin's National Malaria Control Programme (NMCP) organized a nationwide mass distribution campaign of LLINs throughout the country. Following this intervention, it was important to assess whether the level of susceptibility of malaria vectors to insecticides had remained the same as compared to the pre-intervention period. The current study investigated this.

Methods: Larval collections were conducted in Ifangni, Sakété, Pobè and Kétou districts located in Plateau department, Southeastern Benin before (2009) and after (2012-2013) LLIN distribution. Anopheles gambiae sensu lato (s.l.) larvae from the 4 study districts were reared to adulthood and WHO susceptibility tests were conducted. The insecticides tested were deltamethrin (0.05%), permethrin (0.75%), bendiocarb (0.1%) and DDT (4%). Molecular species identification as well as, the characterization of the kdr L1014F mutation were also performed in the An. gambiae s.l. complex using PCR method.

Results: Overall, a significant decrease in mortality rates of An. gambiae s.l. to deltamethrin (0.05%), permethrin (0.75%) and DDT (4%) was observed post-LLIN distribution, respectively: (100% vs 80.9%, p < 0.0001), (77.5% vs 70%, p = 0.01) and, (47.8% vs 4.4%, p < 0.0001). By contrast, susceptibility of vectors to bendiocarb (0.1%) remained the same (100% mortality in the WHO susceptibility tube tests) pre- and post-intervention. An increase in the kdr L1014F frequency was observed post-LLIN distribution [F(kdr) = 0.91)] compared to the pre-intervention period [F(kdr) = 0.56], p < 0.0001. Anopheles coluzzii and An. gambiae were the two molecular species identified in the study area.

Conclusion: The decrease susceptibility to pyrethroids and DDT as well as, the increase in the frequency of the kdr L1014F mutation after the intervention stressed at the time, the need for the development and implementation of effective insecticide resistance management strategies. At present, an update of the vectors resistance status in the area is also necessary for decision-making.

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Yovogan B, Sovi A, Padonou G, Adoha C, Akinro B, Chitou S PLoS One. 2021; 16(5):e0251742.

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