Expression of Oxytocin Receptor in the Rat Superior Cervical Ganglion After Myocardial Infarction

Overview

Journal Int J Clin Exp Pathol

Specialty Pathology

Date 2020 Jan 16

PMID 31938160

Citations 4

Authors

Renliang Yu

Fan Wang

Jie Yin

Yugen Shi

Yu Wang

Shengnan Wen

Hesheng Hu

Suhua Yan

Affiliations

Soon will be listed here.

Abstract

Background: Myocardial infarction (MI) accompanied with abnormal sympathetic innervation, meanwhile, some studies have revealed the oxytocin (OT) and its receptor (OTR) have a relationship with MI and sympathetic system. It is assumed that OT has an close relationship with superior cervical ganglion (SCG), but the existence of oxytocin receptors in SCG has not been well clarified.

Objective: Our research aims to explore the expression of OTR in SCG in the setting of MI.

Methods And Results: MI was induced by coronary artery ligation. Rats were randomly assigned to 2 groups: control, MI. The expression of OTR was measured by Western blotting. Distribution of OTR in SCG was investigated by immunofluorescence. Retrograde tracing test revealed the sprouting of tyrosine hydroxylase (TH: the markers of sensory afferent fibers) from cardiac to SCG neurons. The double-immunofluorescence evidence showed that OTR was co-localized and concomitantly changed with TH and the retrograde neuronal labeling from the cardiac afferent nerves. By Western blotting, the protein of OTR in the MI group was higher than those of the control group.

Conclusions: The expression of OTR in SCG after experimental myocardial infarction group was enhanced, suggesting the involvement of OTR in SCG may play a role in the transmission of sympathetic responses after MI.

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