Transcriptome Analysis Identifies Plasmodiophora Brassicae Secondary Infection Effector Candidates

Overview

Journal J Eukaryot Microbiol

Publisher Wiley

Specialties Microbiology
Parasitology

Date 2020 Jan 12

PMID 31925980

Citations 19

Authors

Edel Perez-Lopez

Md Musharaf Hossain

Jiangying Tu

Matthew Waldner

Christopher D Todd

Anthony J Kusalik

Yangdou Wei

Peta C Bonham-Smith

Affiliations

Soon will be listed here.

Abstract

Plasmodiophora brassicae (Wor.) is an obligate intracellular plant pathogen affecting Brassicas worldwide. Identification of effector proteins is key to understanding the interaction between P. brassicae and its susceptible host plants. To date, there is very little information available on putative effector proteins secreted by P. brassicae during a secondary infection of susceptible host plants, resulting in root gall production. A bioinformatics pipeline approach to RNA-Seq data from Arabidopsis thaliana (L.) Heynh. root tissues at 17, 20, and 24 d postinoculation (dpi) identified 32 small secreted P. brassicae proteins (SSPbPs) that were highly expressed over this secondary infection time frame. Functional signal peptides were confirmed for 31 of the SSPbPs, supporting the accuracy of the pipeline designed to identify secreted proteins. Expression profiles at 0, 2, 5, 7, 14, 21, and 28 dpi verified the involvement of some of the SSPbPs in secondary infection. For seven of the SSPbPs, a functional domain was identified using Blast2GO and 3D structure analysis and domain functionality was confirmed for SSPbP22, a kinase localized to the cytoplasm and nucleus.

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