E-test Versus Agar Dilution for Antibiotic Susceptibility Testing of Helicobacter Pylori: a Comparison Study

Overview

Journal BMC Res Notes

Publisher Biomed Central

Specialties Biology
General Medicine

Date 2020 Jan 12

PMID 31924273

Citations 22

Authors

Muhammad Miftahussurur

Kartika Afrida Fauzia

Iswan Abbas Nusi

Poernomo Boedi Setiawan

Ari Fahrial Syam

Langgeng Agung Waskito

Dalla Doohan

Neneng Ratnasari

Ali Khomsan

I Ketut Adnyana

Junko Akada

Yoshio Yamaoka

Affiliations

Soon will be listed here.

Abstract

Objective: For evaluating the antibiotic resistance of Helicobacter pylori, the agar dilution method is the gold standard; however, using this method in daily practice is laborious. E-test has been proposed to be an uncomplicated method. This study was aimed at validating the E-test and detecting the presence of any bias between the agar dilution method and E-test.

Results: The agar dilution method and E-test were performed using five antibiotics for 72 strains of H. pylori obtained from clinical patients in Indonesia. The E-test's results showed a higher prevalence of resistance to all the antibiotics tested but the difference was not significant. Results showed high essential agreement (> 90.0%) for all the antibiotics, but only 84.7% for metronidazole. The agreement for MIC value was acceptable for levofloxacin, clarithromycin, and metronidazole. For amoxicillin, it showed only fair agreement (0.25) by the Kappa analysis and significant difference by Passing-Bablok regression. Even though some discrepancies were found, the E-test has an acceptable agreement for levofloxacin, metronidazole, tetracycline, and clarithromycin but further confirmation may be necessary for amoxicillin.

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