LncRNA PCAT-1 Promoted ESCC Progression Via Regulating ANXA10 Expression by Sponging MiR-508-3p

Overview

Journal Cancer Manag Res

Publisher Dove Medical Press

Specialty Oncology

Date 2020 Jan 11

PMID 31920393

Citations 13

Authors

Bao Zang

Jianqiang Zhao

Chen Chen

Affiliations

Soon will be listed here.

Abstract

Background: Given the poor prognosis of metastatic esophageal squamous cell carcinoma (ESCC) patients, molecular mechanisms underlying the progression and metastasis of ESCC are highly desired in the scientific community. Prostate cancer associated transcript-1 (PCAT-1) is a lncRNA up-regulated in major types of cancers and is associated with the poor prognosis of cancer patients. This study aimed to understand the expression and role of PCAT-1 in the progression and metastasis of ESCC and to identify the potential lncRNA-miRNA interactions and signaling pathways underlying the mechanisms of action of PCAT-1 in ESCC.

Materials And Methods: Gene expression levels were determined by qRT-PCR; protein levels were determined by Western blot assay; cell proliferation, invasion and migration were determined by CCK-8, Transwell invasion and wound healing assays, respectively; in vivo tumor growth was evaluated by xenograft nude mice model.

Results: Our data showed the up-regulation of PCAT-1 in different human ESCC cell lines and in clinical ESCC tissues. Knockdown of PCAT-1 in ESCC cells significantly inhibited the proliferation, invasion and migration of the cancer cells. Moreover, we showed the interactions between PCAT-1 and miR-508-3p and demonstrated that PCAT-1 was able to repress miR-508-3p expression in ESCC cells via acting as a competing endogenous RNA. Besides, Annexin A10 (ANXA10) was identified to be the downstream target of the PCAT-1 and miR-508-3p interactions.

Conclusion: This study demonstrated the functional role of PCAT-1 in promoting the proliferation, invasion and migration of ESCC cells. We also identified a PCAT-1/miR-508-3p/ANXA10 axis in mediating the promoting role of PCAT-1 in the progression of ESCC. The findings provide experimental evidence to support lncRNA PCAT-1 as a potential therapeutic target of ESCC.

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References

Yang Z, Zhao S, Zhou X, Zhao H, Jiang X . PCAT-1: A pivotal oncogenic long non-coding RNA in human cancers. Biomed Pharmacother. 2018; 110:493-499. DOI: 10.1016/j.biopha.2018.12.014. View

Qiao L, Liu X, Tang Y, Zhao Z, Zhang J, Feng Y . Down regulation of the long non-coding RNA PCAT-1 induced growth arrest and apoptosis of colorectal cancer cells. Life Sci. 2017; 188:37-44. DOI: 10.1016/j.lfs.2017.08.024. View

Hu T, Li Z, Gao C, Cho C . Mechanisms of drug resistance in colon cancer and its therapeutic strategies. World J Gastroenterol. 2016; 22(30):6876-89. PMC: 4974586. DOI: 10.3748/wjg.v22.i30.6876. View

Zhao B, Hou X, Zhan H . Long non-coding RNA PCAT-1 over-expression promotes proliferation and metastasis in non-small cell lung cancer cells. Int J Clin Exp Med. 2016; 8(10):18482-7. PMC: 4694356. View

Liu B, Pan C, Yao G, Wei K, Xia Y, Chen Y . The long non-coding RNA AK001796 contributes to tumor growth via regulating expression of p53 in esophageal squamous cell carcinoma. Cancer Cell Int. 2018; 18:38. PMC: 5857070. DOI: 10.1186/s12935-018-0537-8. View

Hu T, Wang L, Zhang L, Lu L, Shen J, Chan R . Sensitivity of apoptosis-resistant colon cancer cells to tanshinones is mediated by autophagic cell death and p53-independent cytotoxicity. Phytomedicine. 2015; 22(5):536-44. DOI: 10.1016/j.phymed.2015.03.010. View

Munksgaard P, Mansilla F, Brems Eskildsen A, Fristrup N, Birkenkamp-Demtroder K, Ulhoi B . Low ANXA10 expression is associated with disease aggressiveness in bladder cancer. Br J Cancer. 2011; 105(9):1379-87. PMC: 3241563. DOI: 10.1038/bjc.2011.404. View

Zhang F, Wan M, Xu Y, Li Z, Leng K, Kang P . Long noncoding RNA PCAT1 regulates extrahepatic cholangiocarcinoma progression via the Wnt/β-catenin-signaling pathway. Biomed Pharmacother. 2017; 94:55-62. DOI: 10.1016/j.biopha.2017.07.025. View

Hu T, To K, Wang L, Zhang L, Lu L, Shen J . Reversal of P-glycoprotein (P-gp) mediated multidrug resistance in colon cancer cells by cryptotanshinone and dihydrotanshinone of Salvia miltiorrhiza. Phytomedicine. 2014; 21(11):1264-72. DOI: 10.1016/j.phymed.2014.06.013. View

10.

Shi W, Wu Q, Li S, Yang T, Liu Z, Tong Y . Upregulation of the long noncoding RNA PCAT-1 correlates with advanced clinical stage and poor prognosis in esophageal squamous carcinoma. Tumour Biol. 2015; 36(4):2501-7. DOI: 10.1007/s13277-014-2863-3. View

11.

Chen W, Zheng R, Baade P, Zhang S, Zeng H, Bray F . Cancer statistics in China, 2015. CA Cancer J Clin. 2016; 66(2):115-32. DOI: 10.3322/caac.21338. View

12.

Zhang X, Zhang Y, Mao Y, Ma X . The lncRNA PCAT1 is correlated with poor prognosis and promotes cell proliferation, invasion, migration and EMT in osteosarcoma. Onco Targets Ther. 2018; 11:629-638. PMC: 5797453. DOI: 10.2147/OTT.S152063. View

13.

Enzinger P, Mayer R . Esophageal cancer. N Engl J Med. 2003; 349(23):2241-52. DOI: 10.1056/NEJMra035010. View

14.

Xu W, Chang J, Du X, Hou J . Long non-coding RNA PCAT-1 contributes to tumorigenesis by regulating FSCN1 via miR-145-5p in prostate cancer. Biomed Pharmacother. 2017; 95:1112-1118. DOI: 10.1016/j.biopha.2017.09.019. View

15.

Kung J, Colognori D, Lee J . Long noncoding RNAs: past, present, and future. Genetics. 2013; 193(3):651-69. PMC: 3583990. DOI: 10.1534/genetics.112.146704. View

16.

Zhang D, Cao J, Zhong Q, Zeng L, Cai C, Lei L . Long noncoding RNA PCAT-1 promotes invasion and metastasis via the miR-129-5p-HMGB1 signaling pathway in hepatocellular carcinoma. Biomed Pharmacother. 2017; 95:1187-1193. DOI: 10.1016/j.biopha.2017.09.045. View

17.

Wu S, Zhang W, He Z, Sun J, Chen Y, Guo L . Sites of metastasis and overall survival in esophageal cancer: a population-based study. Cancer Manag Res. 2017; 9:781-788. PMC: 5723120. DOI: 10.2147/CMAR.S150350. View

18.

Prensner J, Iyer M, Balbin O, Dhanasekaran S, Cao Q, Brenner J . Transcriptome sequencing across a prostate cancer cohort identifies PCAT-1, an unannotated lincRNA implicated in disease progression. Nat Biotechnol. 2011; 29(8):742-9. PMC: 3152676. DOI: 10.1038/nbt.1914. View

19.

Su M, Xiao Y, Ma J, Cao D, Zhou Y, Wang H . Long non-coding RNAs in esophageal cancer: molecular mechanisms, functions, and potential applications. J Hematol Oncol. 2018; 11(1):118. PMC: 6142629. DOI: 10.1186/s13045-018-0663-8. View

20.

Yan T, Yang H, Jiang J, Lu S, Peng C, Que H . Prognostic significance of long non-coding RNA PCAT-1 expression in human hepatocellular carcinoma. Int J Clin Exp Pathol. 2015; 8(4):4126-31. PMC: 4466989. View