» Articles » PMID: 31919299

Kinesin-13 and Kinesin-8 Function During Cell Growth and Division in the Moss

Overview
Journal Plant Cell
Specialties Biology
Cell Biology
Date 2020 Jan 11
PMID 31919299
Citations 17
Authors
Affiliations
Soon will be listed here.
Abstract

Kinesin-13 and Kinesin-8 are well-known microtubule (MT) depolymerases that regulate MT length and chromosome movement in animal mitosis. While much is unknown about plant Kinesin-8, Arabidopsis () and rice () Kinesin-13 have been shown to depolymerize MTs in vitro. However, the mitotic function of both kinesins has yet to be determined in plants. Here, we generated complete null mutants of and in moss (). Both kinesins were found to be nonessential for viability, but the knockout (KO) line had increased mitotic duration and reduced spindle length, whereas the KO line did not display obvious mitotic defects. Surprisingly, spindle MT poleward flux, which is mediated by Kinesin-13 in animals, was retained in the absence of Kinesin-13. MT depolymerase activity was not detectable for either kinesin in vitro, while MT catastrophe-inducing activity (Kinesin-13) or MT gliding activity (Kinesin-8) was observed. Interestingly, both KO lines showed waviness in their protonema filaments, which correlated with positional instability of the MT foci in their tip cells. Taken together, the results suggest that plant Kinesin-13 and Kinesin-8 have diverged in both mitotic function and molecular activity, acquiring roles in regulating MT foci positioning for directed tip growth.

Citing Articles

From the archives: Roles of microtubule-associated proteins in organelle movement, tip growth, and phragmoplast architecture.

Kolli R Plant Cell. 2024; 37(1.

PMID: 39691058 PMC: 11708835. DOI: 10.1093/plcell/koae331.


KIF18B: an important role in signaling pathways and a potential resistant target in tumor development.

Chen S, Yu B, DU G, Huang T, Zhang N, Fu N Discov Oncol. 2024; 15(1):430.

PMID: 39259333 PMC: 11390998. DOI: 10.1007/s12672-024-01330-4.


Genome-wide identification of the key kinesin genes during fiber and boll development in upland cotton (Gossypium hirsutum L.).

Zhu H, Xu J, Yu K, Wu J, Xu H, Wang S Mol Genet Genomics. 2024; 299(1):38.

PMID: 38517563 DOI: 10.1007/s00438-024-02093-x.


The third Japan-U.S. symposium on motor proteins and associated single-molecule biophysics.

Shima T, Hayashi K Biophys Physicobiol. 2024; 20(4):e200037.

PMID: 38344028 PMC: 10850461. DOI: 10.2142/biophysico.bppb-v20.0037.


Genome-wide identification of the key Kinesin genes during fiber and boll development in upland cotton (Gossypium hirsutum L).

Zhu H, Xu J, Yu K, Wu J, Xu H, Wang S Mol Genet Genomics. 2024; 299(1):2.

PMID: 38200363 DOI: 10.1007/s00438-023-02087-1.


References
1.
Niwa S, Nakajima K, Miki H, Minato Y, Wang D, Hirokawa N . KIF19A is a microtubule-depolymerizing kinesin for ciliary length control. Dev Cell. 2012; 23(6):1167-75. DOI: 10.1016/j.devcel.2012.10.016. View

2.
Moores C, Yu M, Guo J, Beraud C, Sakowicz R, Milligan R . A mechanism for microtubule depolymerization by KinI kinesins. Mol Cell. 2002; 9(4):903-9. DOI: 10.1016/s1097-2765(02)00503-8. View

3.
Maney T, Wagenbach M, Wordeman L . Molecular dissection of the microtubule depolymerizing activity of mitotic centromere-associated kinesin. J Biol Chem. 2001; 276(37):34753-8. DOI: 10.1074/jbc.M106626200. View

4.
Moriwaki T, Goshima G . Five factors can reconstitute all three phases of microtubule polymerization dynamics. J Cell Biol. 2016; 215(3):357-368. PMC: 5100292. DOI: 10.1083/jcb.201604118. View

5.
Mayr M, Hummer S, Bormann J, Gruner T, Adio S, Woehlke G . The human kinesin Kif18A is a motile microtubule depolymerase essential for chromosome congression. Curr Biol. 2007; 17(6):488-98. DOI: 10.1016/j.cub.2007.02.036. View