Erythromyeloid Progenitors Give Rise to a Population of Osteoclasts That Contribute to Bone Homeostasis and Repair

Overview

Journal Nat Cell Biol

Specialty Cell Biology

Date 2020 Jan 8

PMID 31907410

Citations 86

Authors

Yasuhito Yahara

Tomasa Barrientos

Yuning J Tang

Vijitha Puviindran

Puviindran Nadesan

Hongyuan Zhang

Jason R Gibson

Simon G Gregory

Yarui Diao

Yu Xiang

Yawar J Qadri

Tomokazu Souma

Mari L Shinohara

Benjamin A Alman

Affiliations

Soon will be listed here.

Abstract

Osteoclasts are multinucleated cells of the monocyte/macrophage lineage that degrade bone. Here, we used lineage tracing studies-labelling cells expressing Cx3cr1, Csf1r or Flt3-to identify the precursors of osteoclasts in mice. We identified an erythromyeloid progenitor (EMP)-derived osteoclast precursor population. Yolk-sac macrophages of EMP origin produced neonatal osteoclasts that can create a space for postnatal bone marrow haematopoiesis. Furthermore, EMPs gave rise to long-lasting osteoclast precursors that contributed to postnatal bone remodelling in both physiological and pathological settings. Our single-cell RNA-sequencing data showed that EMP-derived osteoclast precursors arose independently of the haematopoietic stem cell (HSC) lineage and the data from fate tracking of EMP and HSC lineages indicated the possibility of cell-cell fusion between these two lineages. Cx3cr1 yolk-sac macrophage descendants resided in the adult spleen, and parabiosis experiments showed that these cells migrated through the bloodstream to the remodelled bone after injury.

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