Antibacterial Activity of Some Flavonoids and Organic Acids Widely Distributed in Plants

Overview

Journal J Clin Med

Specialty General Medicine

Date 2020 Jan 8

PMID 31906141

Citations 128

Authors

Artur Adamczak

Marcin Ozarowski

Tomasz M Karpinski

Affiliations

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Abstract

Among natural substances widespread in fruits, vegetables, spices, and medicinal plants, flavonoids and organic acids belong to the promising groups of bioactive compounds with strong antioxidant and anti-inflammatory properties. The aim of the present work was to evaluate the antibacterial activity of 13 common flavonoids (flavones, flavonols, flavanones) and 6 organic acids (aliphatic and aromatic acids). The minimal inhibitory concentrations (MICs) of selected plant substances were determined by the micro-dilution method using clinical strains of four species of pathogenic bacteria. All tested compounds showed antimicrobial properties, but their biological activity was moderate or relatively low. Bacterial growth was most strongly inhibited by salicylic acid (MIC = 250-500 μg/mL). These compounds were generally more active against Gram-negative bacteria: and than Gram-positive ones: and . An analysis of the antibacterial effect of flavone, chrysin, apigenin, and luteolin showed that the presence of hydroxyl groups in the phenyl rings A and B usually did not influence on the level of their activity. A significant increase in the activity of the hydroxy derivatives of flavone was observed only for . Similarly, the presence and position of the sugar group in the flavone glycosides generally had no effect on the MIC values.

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References

Enogieru A, Haylett W, Hiss D, Bardien S, Ekpo O . Rutin as a Potent Antioxidant: Implications for Neurodegenerative Disorders. Oxid Med Cell Longev. 2018; 2018:6241017. PMC: 6040293. DOI: 10.1155/2018/6241017. View

Gupta P, Birdi T . Development of botanicals to combat antibiotic resistance. J Ayurveda Integr Med. 2017; 8(4):266-275. PMC: 5747506. DOI: 10.1016/j.jaim.2017.05.004. View

Basile A, Giordano S, Lopez-Saez J, Cobianchi R . Antibacterial activity of pure flavonoids isolated from mosses. Phytochemistry. 2000; 52(8):1479-82. DOI: 10.1016/s0031-9422(99)00286-1. View

Zheng J, Huang C, Yang B, Kallio H, Liu P, Ou S . Regulation of phytochemicals in fruits and berries by environmental variation-Sugars and organic acids. J Food Biochem. 2019; 43(6):e12642. DOI: 10.1111/jfbc.12642. View

Lin H, Huang C . Characterization of flavonol inhibition of DnaB helicase: real-time monitoring, structural modeling, and proposed mechanism. J Biomed Biotechnol. 2012; 2012:735368. PMC: 3468084. DOI: 10.1155/2012/735368. View

Ekambaram S, Perumal S, Balakrishnan A, Marappan N, Gajendran S, Viswanathan V . Antibacterial synergy between rosmarinic acid and antibiotics against methicillin-resistant . J Intercult Ethnopharmacol. 2016; 5(4):358-363. PMC: 5061478. DOI: 10.5455/jice.20160906035020. View

Smiljkovic M, Stanisavljevic D, Stojkovic D, Petrovic I, Marjanovic Vicentic J, Popovic J . Apigenin-7-O-glucoside versus apigenin: Insight into the modes of anticandidal and cytotoxic actions. EXCLI J. 2017; 16:795-807. PMC: 5547395. DOI: 10.17179/excli2017-300. View

Rammohan A, Bhaskar B, Venkateswarlu N, Rao V, Gunasekar D, Zyryanov G . Isolation of flavonoids from the flowers of Rhynchosia beddomei Baker as prominent antimicrobial agents and molecular docking. Microb Pathog. 2019; 136:103667. DOI: 10.1016/j.micpath.2019.103667. View

Chuang S, Lin Y, Lin C, Wang P, Chen E, Fang J . Elucidating the Skin Delivery of Aglycone and Glycoside Flavonoids: How the Structures Affect Cutaneous Absorption. Nutrients. 2017; 9(12). PMC: 5748754. DOI: 10.3390/nu9121304. View

10.

Kerasioti E, Apostolou A, Kafantaris I, Chronis K, Kokka E, Dimitriadou C . Polyphenolic Composition of , and Extracts and Assessment of Their Antioxidant Activity in Human Endothelial Cells. Antioxidants (Basel). 2019; 8(4). PMC: 6523071. DOI: 10.3390/antiox8040092. View

11.

de Lima Yamaguchi K, Pereira L, Lamarao C, Lima E, Veiga-Junior V . Amazon acai: chemistry and biological activities: a review. Food Chem. 2015; 179:137-51. DOI: 10.1016/j.foodchem.2015.01.055. View

12.

Chen C, Huang C . Inhibition of Klebsiella pneumoniae DnaB helicase by the flavonol galangin. Protein J. 2011; 30(1):59-65. DOI: 10.1007/s10930-010-9302-0. View

13.

Goncalves S, Moreira E, Grosso C, Andrade P, Valentao P, Romano A . Phenolic profile, antioxidant activity and enzyme inhibitory activities of extracts from aromatic plants used in Mediterranean diet. J Food Sci Technol. 2017; 54(1):219-227. PMC: 5305718. DOI: 10.1007/s13197-016-2453-z. View

14.

Karpinski T, Adamczak A . Fucoxanthin-An Antibacterial Carotenoid. Antioxidants (Basel). 2019; 8(8). PMC: 6720875. DOI: 10.3390/antiox8080239. View

15.

Nabavi S, Habtemariam S, Ahmed T, Sureda A, Daglia M, Sobarzo-Sanchez E . Polyphenolic Composition of Crataegus monogyna Jacq.: From Chemistry to Medical Applications. Nutrients. 2015; 7(9):7708-28. PMC: 4586556. DOI: 10.3390/nu7095361. View

16.

Blaskovich M, Elliott A, Kavanagh A, Ramu S, Cooper M . In vitro Antimicrobial Activity of Acne Drugs Against Skin-Associated Bacteria. Sci Rep. 2019; 9(1):14658. PMC: 6787063. DOI: 10.1038/s41598-019-50746-4. View

17.

Naz S, Imran M, Rauf A, Orhan I, Shariati M, Iahtisham-Ul-Haq . Chrysin: Pharmacological and therapeutic properties. Life Sci. 2019; 235:116797. DOI: 10.1016/j.lfs.2019.116797. View

18.

Mani R, Natesan V . Chrysin: Sources, beneficial pharmacological activities, and molecular mechanism of action. Phytochemistry. 2017; 145:187-196. DOI: 10.1016/j.phytochem.2017.09.016. View

19.

Ozarowski M, Mikolajczak P, Piasecka A, Kachlicki P, Kujawski R, Bogacz A . Influence of the Melissa officinalis Leaf Extract on Long-Term Memory in Scopolamine Animal Model with Assessment of Mechanism of Action. Evid Based Complement Alternat Med. 2016; 2016:9729818. PMC: 4864554. DOI: 10.1155/2016/9729818. View

20.

Xie Y, Chen J, Xiao A, Liu L . Antibacterial Activity of Polyphenols: Structure-Activity Relationship and Influence of Hyperglycemic Condition. Molecules. 2017; 22(11). PMC: 6150409. DOI: 10.3390/molecules22111913. View