Antibacterial Synergy Between Rosmarinic Acid and Antibiotics Against Methicillin-resistant

Aim/background: Medicinal plants have ability to resist microorganisms by synthesizing secondary metabolites such as phenols. Rosmarinic acid (RA) is a phenylpropanoid widely distributed in plants and well known as therapeutic and cosmetic agent. Methicillin-resistant (MRSA) which is resistant to all kinds of β-lactams, threatens even most potent antibiotics. To improve the efficiency of antibiotics against multi-drug resistant bacteria and to reduce the antibiotic dose, the antibacterial activity and the synergistic effect of RA with standard antibiotics against and MRSA was investigated.

Materials And Methods: Antibacterial activity of RA against and a clinical isolate of MRSA was evaluated by agar well diffusion method. Minimum inhibitory concentration (MIC) of RA was determined by broth dilution method. Synergism of RA with various antibiotics against and MRSA was studied by broth checkerboard method and time-kill kinetic assay. Effect of RA on microbial surface components recognizing adhesive matrix molecules (MSCRAMM's) of and MRSA was studied using sodium dodecyl sulfate - polyacrylamide gel electrophoresis.

Results: MIC of RA was found to be 0.8 and 10 mg/ml against and MRSA, respectively. RA was synergistic with vancomycin, ofloxacin, and amoxicillin against and only with vancomycin against MRSA. The time-kill analysis revealed that synergistic combinations were a more effective than individual antibiotics. MSCRAMM's protein expression of and MRSA was markedly suppressed by RA + vancomycin combination rather than RA alone.

Conclusion: The synergistic effects of RA with antibiotics were observed against and MRSA. RA showed inhibitory effect on the surface proteins MSCRAMM's. Even though RA was shown to exhibit a synergistic effect with antibiotics, the MIC was found to be higher. Thus, further studies on increasing the efficacy of RA can develop it as an adjuvant for antibiotics.