High Rates of Aneuploidy, Mosaicism and Abnormal Morphokinetic Development in Cases with Low Sperm Concentration

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2020 Jan 5

PMID 31901112

Citations 17

Authors

Semra Kahraman

Yucel Sahin

Hakan Yelke

Yesim Kumtepe

Mehmet A Tufekci

Cigdem C Yapan

Mesut Yesil

Murat Cetinkaya

Affiliations

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Abstract

Purpose: The aim of our study was to evaluate the impact of severe male infertility (SMF) on the chromosomal status of embryos and any possible correlation between chromosomal status and embryo morphokinetics in younger women using data obtained from comprehensive preimplantation genetic tests.

Methods: The trial was conducted in an ART and Reproductive Genetics Centre between 2011 and 2018. A total of 326 cycles in cases with SMF where the female partner's age was ≤ 35 years were evaluated. SMF is defined as sperm concentration below 5 mil/ml (million per milliliter) and divided into three subgroups according to sperm concentrations: 1-5 mil/ml, < 1mil/ml and testicular sperm. The control group of 190 cycles had normal sperm parameters.

Results: Significantly lower chromosomal euploidy rates were found in the testicular sperm group compared with the normal sperm controls when the female age was ≤ 35 years. In SMF, statistically significantly affected chromosomes were 2, 10, 11, 17, 21 and sex chromosomes. The mosaicism and abnormal morphokinetic development rates were higher in the SMF group than in control group, and this difference was significant when testicular sperm was used.

Conclusion: Lower euploidy rates, higher mosaicism rates and a higher incidence of abnormal morphokinetic development were observed in cases with testicular sperm with female partners ≤ 35 years compared with normal sperm controls. These findings suggest that PGT-A may be advisable in severe male infertility cases. Furthermore, the correlation between morphokinetics and chromosomal status was greatly reduced or absent in these most severe forms of male infertility, thus the need for new morphokinetic models.

Citing Articles

Preimplantation Genetic Testing for Aneuploidy in Fertilization Using Comprehensive Chromosome Screening: A Systematic Review and Meta-Analysis.

Taskin O, Hochberg A, Tan J, Adye-White L, Albert A, Tan S Int J Fertil Steril. 2024; 18(3):185-194.

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Are there associations between clinical and embryological factors with pregnancy loss following transfer of a single euploid embryo?.

Yuksel B, Ozer G, Duzguner I, Akca A, Bio Y, Bio H Reprod Fertil. 2024; .

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Exploring the efficacy and beneficial population of preimplantation genetic testing for aneuploidy start from the oocyte retrieval cycle: a real-world study.

Ma S, Liao J, Zhang S, Yang X, Hocher B, Tan J J Transl Med. 2023; 21(1):779.

PMID: 37919732 PMC: 10623718. DOI: 10.1186/s12967-023-04641-2.

Factors associated with embryo mosaicism: a systematic review and meta-analysis.

Cascales A, Morales R, Castro A, Ortiz J, Lledo B, Ten J J Assist Reprod Genet. 2023; 40(10):2317-2324.

PMID: 37592098 PMC: 10504166. DOI: 10.1007/s10815-023-02914-9.

IVF outcomes following ICSI cycles using testicular sperm in obstructive (OA) vs. non-obstructive azoospermia (NOA) and the impact of maternal and paternal age: a SART CORS data registry.

Sadek S, Matitashvili T, Sharaf Alddin R, Morshedi B, Ramadan H, Dodani S J Assist Reprod Genet. 2023; 40(3):627-637.

PMID: 36662354 PMC: 10033785. DOI: 10.1007/s10815-023-02726-x.

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