Second-generation Probiotics Producing IL-22 Increase Survival of Mice After Total Body Irradiation

Overview

Journal In Vivo

Specialty Oncology

Date 2019 Dec 29

PMID 31882461

Citations 16

Authors

Xichen Zhang

Renee Fisher

Wen Hou

Donna Shields

Michael W Epperly

Hong Wang

Liang Wei

Brian J Leibowitz

Jian Yu

Laura M Alexander

Jan-Peter van Pijkeren

Simon Watkins

Peter Wipf

Joel S Greenberger

Affiliations

Soon will be listed here.

Abstract

Background/aim: Intestinal damage induced by total body irradiation (TBI) reduces leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5)-expressing stem cells, goblet, and Paneth cells, breaching the epithelial lining, and facilitating bacterial translocation, sepsis, and death.

Materials And Methods: Survival was measured after TBI in animals that received wild-type or recombinant bacteria producing interleukin-22 (IL-22). Changes in survival due to microbially delivered IL-22 were measured. Lactobacillus reuteri producing IL-22, or Escherichia coli-IL-22 were compared to determine which delivery system is better.

Results: C57BL/6 mice receiving IL-22 probiotics at 24 h after 9.25 Gy TBI, demonstrated green fluorescent protein-positive bacteria in the intestine, doubled the number of Lgr5+ intestinal stem cells, and increased 30-day survival. Bacteria were localized to the jejunum, ileum, and colon.

Conclusion: Second-generation probiotics appear to be valuable for mitigation of TBI, and radiation protection during therapeutic total abdominal irradiation.

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