Genetic Inversion in the Formation of an Hfr Strain from a Temperature-sensitive F' Gal Strain

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1977 Jan 1

PMID 318642

Citations 4

Authors

P L Bergquist

A F Jamieson

Affiliations

Soon will be listed here.

Abstract

An Hfr strain (PB15) that carries a duplicated copy of the galactose operon genes flanking the integrated sex factor is unusually stable since it does not show excision of the repeated deoxyribonucleic acid segment. The right-hand galactose operon is in the normal orientation. Deletion mutations that eliminate the right-hand galactose genes, the sex factor, and some of the left-hand operon have been isolated. Mutants believed to have their left-hand galactose operon inverted were able to be induced for galactose epimerase synthesis by D-fucose but did not show escape synthesis on induction of bacteriophage lambda. Ribonucleic acid specific for the galactose operon was isolated after induction of lysogenic strains presumed to carry the galactose operon in the normal and inverted orientation. Hybridization to the isolated left and right strands of lambdapgal showed that the noninformational strand of the left-hand galactose operon of the deletion mutant of PB15 was transcribed on escape induction. These results show that inversion has occurred.

Citing Articles

Genetic factors affecting recovery of nonpoint mutations in the region of a gene coding for ornithine transcarbamylase: involvement of both the F factor in its chromosomal state and the recA gene.

JESSOP A, Glansdorff N Genetics. 1980; 96(4):779-99.

PMID: 7021315 PMC: 1219301. DOI: 10.1093/genetics/96.4.779.

A naturally occurring large chromosomal inversion in Escherichia coli K12.

Xia X, Enomoto M Mol Gen Genet. 1986; 205(2):376-9.

PMID: 3543622 DOI: 10.1007/BF00430454.

Mapping by transposons of the inversion termini in Escherichia coli K-12 strain 1485IN.

Enomoto M, Komoda Y, Tominaga A Genetics. 1991; 129(3):631-8.

PMID: 1661251 PMC: 1204730. DOI: 10.1093/genetics/129.3.631.

Inversion in the lactose region of Escherichia coli K-12.

Savic D J Bacteriol. 1979; 140(2):311-9.

PMID: 387729 PMC: 216651. DOI: 10.1128/jb.140.2.311-319.1979.

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