Mapping by Transposons of the Inversion Termini in Escherichia Coli K-12 Strain 1485IN

Overview

Journal Genetics

Publisher Oxford University Press

Specialty Genetics

Date 1991 Nov 1

PMID 1661251

Authors

M Enomoto

Y Komoda

A Tominaga

Affiliations

Soon will be listed here.

Abstract

Strain 1485IN carries a chromosomal inversion which corresponds to 35% of the chromosome and includes proC, trp and his genes. The termini of the inversion lie between the lac and proC loci and between his and cdd of the normal strain. Using Tn10 and Tn5 in transduction crosses between the normal and inversion strains, the termini were mapped to sites located approximately 0.25 min and 1.6 min away from proC and his, respectively within a region of roughly 4 kb long. The crosses where the normal strains carrying Tn10 near the terminus are donors and the inversion strain is a recipient, yielded unusual Tetr His- recombinants, which arose from illegitimate recombination leading to the replacement of a chromosomal his+ region with a transducing fragment carrying proC. Another rearrangement was detected between the normal and inversion strains in a region outside the inverted segment near the cdd locus.

References

Chumley F, Menzel R, Roth J . Hfr formation directed by tn10. Genetics. 1979; 91(4):639-55. PMC: 1216857. DOI: 10.1093/genetics/91.4.639. View

Berg C, Curtiss 3rd R . Transposition derivatives of an Hfr strain of Escherichia coli K-12. Genetics. 1967; 56(3):503-25. PMC: 1211636. DOI: 10.1093/genetics/56.3.503. View

LEDERBERG E, LEDERBERG J . Genetic Studies of Lysogenicity in Escherichia Coli. Genetics. 1953; 38(1):51-64. PMC: 1209586. DOI: 10.1093/genetics/38.1.51. View

Segall A, Roth J . Recombination between homologies in direct and inverse orientation in the chromosome of Salmonella: intervals which are nonpermissive for inversion formation. Genetics. 1989; 122(4):737-47. PMC: 1203750. DOI: 10.1093/genetics/122.4.737. View

Kohara Y, Akiyama K, Isono K . The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell. 1987; 50(3):495-508. DOI: 10.1016/0092-8674(87)90503-4. View

Rebollo J, Francois V, Louarn J . Detection and possible role of two large nondivisible zones on the Escherichia coli chromosome. Proc Natl Acad Sci U S A. 1988; 85(24):9391-5. PMC: 282758. DOI: 10.1073/pnas.85.24.9391. View

Hanafusa T, Sakai A, Tominaga A, Enomoto M . Isolation and characterization of Escherichia coli hag operator mutants whose hag48 expression has become repressible by a Salmonella H1 repressor. Mol Gen Genet. 1989; 216(1):44-50. DOI: 10.1007/BF00332229. View

HILL C, Gray J . Effects of chromosomal inversion on cell fitness in Escherichia coli K-12. Genetics. 1988; 119(4):771-8. PMC: 1203463. DOI: 10.1093/genetics/119.4.771. View

Louarn J, Bouche J, Legendre F, Louarn J, PATTE J . Characterization and properties of very large inversions of the E. coli chromosome along the origin-to-terminus axis. Mol Gen Genet. 1985; 201(3):467-76. DOI: 10.1007/BF00331341. View

10.

Bachmann B . Linkage map of Escherichia coli K-12, edition 8. Microbiol Rev. 1990; 54(2):130-97. PMC: 372767. DOI: 10.1128/mr.54.2.130-197.1990. View

11.

Savic D, Romac S, Ehrlich S . Inversion in the lactose region of Escherichia coli K-12: inversion termini map within IS3 elements alpha 3 beta 3 and beta 5 alpha 5. J Bacteriol. 1983; 155(2):943-6. PMC: 217778. DOI: 10.1128/jb.155.2.943-946.1983. View

12.

HILL C, Harnish B . Inversions between ribosomal RNA genes of Escherichia coli. Proc Natl Acad Sci U S A. 1981; 78(11):7069-72. PMC: 349196. DOI: 10.1073/pnas.78.11.7069. View

13.

Faelen M, Toussaint A . Inversion induced by temperature bacteriophage mu-1 in the chromosome of Escherichia coli K-12. J Bacteriol. 1980; 142(2):391-9. PMC: 293983. DOI: 10.1128/jb.142.2.391-399.1980. View

14.

Maloy S, Nunn W . Selection for loss of tetracycline resistance by Escherichia coli. J Bacteriol. 1981; 145(2):1110-1. PMC: 217228. DOI: 10.1128/jb.145.2.1110-1111.1981. View

15.

Bergquist P, Jamieson A . Genetic inversion in the formation of an Hfr strain from a temperature-sensitive F' gal strain. J Bacteriol. 1977; 129(1):282-90. PMC: 234925. DOI: 10.1128/jb.129.1.282-290.1977. View

16.

Kleckner N, Barker D, Ross D, Botstein D . Properties of the translocatable tetracycline-resistance element Tn10 in Escherichia coli and bacteriophage lambda. Genetics. 1978; 90(3):427-61. PMC: 1213899. DOI: 10.1093/genetics/90.3.427. View

17.

HOEKSTRA W, Vis H . Characterization of the E. coli K12 strain AB1157 as impaired in guanine/xanthine metabolism. Antonie Van Leeuwenhoek. 1977; 43(2):199-204. DOI: 10.1007/BF00395674. View

18.

Kleckner N, Roth J, Botstein D . Genetic engineering in vivo using translocatable drug-resistance elements. New methods in bacterial genetics. J Mol Biol. 1977; 116(1):125-59. DOI: 10.1016/0022-2836(77)90123-1. View

19.

Cassio D, Mathien Y, Waller J . Enhanced level and metabolic regulation of methionyl-transfer ribonucleic acid synthetase in different strains of Escherichia coli K-12. J Bacteriol. 1975; 123(2):580-8. PMC: 235763. DOI: 10.1128/jb.123.2.580-588.1975. View

20.

Casse F, PASCAL M, Chippaux M . Comparison between the chromosomal maps of Escherichia coli and Salmonella typhimurium. Length of the inverted segment in the trp region. Mol Gen Genet. 1973; 124(3):253-7. DOI: 10.1007/BF00293096. View