Cell Membrane Localization of Long Chain C24-C30 Fatty Acids in Two Marine Demosponges

Overview

Journal Lipids

Specialty Biochemistry

Date 1988 Aug 1

PMID 3185105

Citations 9

Authors

M P Lawson

J E Thompson

C Djerassi

Affiliations

Soon will be listed here.

Abstract

Subcellular fractionation by differential centrifugation was performed on two previously unstudied marine sponges (Reniera sp. and Pseudaxinyssa sp.) that represent both major subclasses of the Demospongiae. Long chain fatty acids (LCFA) with 24-30 carbon units were found as major constituents of cell membrane isolates of both sponges. Most LCFA were polyunsaturated and were constituents of the phospholipids, which are typical membrane lipids, and in particular the amino-phospholipids. The LCFA composition of phospholipids from whole sponge tissue was shown to provide a reliable indication of the LCFA composition of cell membrane phospholipids in the sponges studied. An unusual triply branched C16 isoprenoid fatty acid, 4,8,12-trimethyltridecanoic acid, also was identified as a cell membrane acid in the sponge Pseudaxinyssa sp.

Citing Articles

Bacterial precursors and unsaturated long-chain fatty acids are biomarkers of North-Atlantic deep-sea demosponges.

de Kluijver A, Nierop K, Morganti T, Bart M, Slaby B, Hanz U PLoS One. 2021; 16(1):e0241095.

PMID: 33503057 PMC: 7840048. DOI: 10.1371/journal.pone.0241095.

Status and perspective of sponge chemosystematics.

Erpenbeck D, Soest R Mar Biotechnol (NY). 2006; 9(1):2-19.

PMID: 16817029 DOI: 10.1007/s10126-005-6109-7.

Unusually high levels of eicosatetraenoic, eicosapentaenoic, and docosahexaenoic fatty acids in Palestinian freshwater sponges.

Dembitsky V, rezanka T Lipids. 1996; 31(6):647-50.

PMID: 8784746 DOI: 10.1007/BF02523836.

The distribution of brominated long-chain fatty acids in sponge and symbiont cell types from the tropical marine sponge Amphimedon terpenensis.

Garson M, Zimmermann M, Battershill C, HOLDEN J, Murphy P Lipids. 1994; 29(7):509-16.

PMID: 7968273 DOI: 10.1007/BF02578249.

Localization of long-chain fatty acids and unconventional sterols in spherulous cells of a marine sponge.

Lawson M, Thompson J, Djerassi C Lipids. 1988; 23(11):1037-48.

PMID: 3237003 DOI: 10.1007/BF02535649.

References

Morrison W, Smith L . PREPARATION OF FATTY ACID METHYL ESTERS AND DIMETHYLACETALS FROM LIPIDS WITH BORON FLUORIDE--METHANOL. J Lipid Res. 1964; 5:600-8. View

Jefferts E, Morales R, Litchfield C . Occurrence of cis-5, cis-9-hexacosadienoic and cis-5, cis-9, cis-19-hexacosatrienoic acids in the marine sponge Microciona prolifera. Lipids. 1974; 9(4):244-7. DOI: 10.1007/BF02532200. View

Ha T, Kokke W, Proudfoot J, Djerassi C, Thompson J . Minor and trace sterols in marine invertebrates 53 (1): Further novel marine sterols resulting from triple and quadruple biomethylation of the cholesterol side-chain. Steroids. 1985; 45(3-4):263-76. DOI: 10.1016/0039-128x(85)90075-3. View

Ayanoglu E, Popov S, Kornprobst J, Aboud-Bichara A, Djerassi C . Phospholipid studies of marine organisms: V(1) new α-methoxy acids fromHigginsia tethyoides. Lipids. 2016; 18(11):830-6. DOI: 10.1007/BF02534643. View

Privett O, Dougherty K, Erdahl W, Stolyhwo A . Studies on the lipid composition of developing soybeans. J Am Oil Chem Soc. 1973; 50(12):516-20. DOI: 10.1007/BF02640523. View

Spurr A . A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res. 1969; 26(1):31-43. DOI: 10.1016/s0022-5320(69)90033-1. View

Morales R, Litchfield C . Unusual C24, C25, C26 and C27 polyunsaturated fatty acids of the marine sponge Microciona prolifera. Biochim Biophys Acta. 1976; 431(2):206-16. DOI: 10.1016/0005-2760(76)90140-5. View

Wong M, Steck P, Gray G . The major mycolic acids of Mycobacterium smegmatis. Characterization of their homologous series. J Biol Chem. 1979; 254(13):5734-40. View

Andersson B . Mass spectrometry of fatty acid pyrrolidides. Prog Chem Fats Other Lipids. 1978; 16:279-308. DOI: 10.1016/0079-6832(78)90048-4. View

10.

Lethias C, Garrone R, Mazzorana M . Fine structures of sponge cell membranes: comparative study with freeze-fracture and conventional thin section methods. Tissue Cell. 1983; 15(4):523-35. DOI: 10.1016/0040-8166(83)90004-6. View

11.

BLIGH E, Dyer W . A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959; 37(8):911-7. DOI: 10.1139/o59-099. View

12.

Wilkinson C . Net primary productivity in coral reef sponges. Science. 1983; 219(4583):410-2. DOI: 10.1126/science.219.4583.410. View

13.

Lawson M, Stoilov I, Thompson J, Djerassi C . Cell membrane localization of sterols with conventional and unusual side chains in two marine demosponges. Lipids. 1988; 23(8):750-4. DOI: 10.1007/BF02536216. View

14.

Lawson M, Bergquist P, Cambie R . The cellular localization of long chain fatty acids in sponges. Tissue Cell. 1986; 18(1):19-26. DOI: 10.1016/0040-8166(86)90004-2. View

15.

Litchfield C, Greenberg A, Noto G, Morales R . Unusually high levels of C24-C30 fatty acids in sponges of the class Demospongiae. Lipids. 1976; 11(7):567-73. DOI: 10.1007/BF02532903. View

16.

Walkup R, Jamieson G, Ratcliff M, Djerassi C . Phospholpid studies of marine organisms: 2.(1) Phospholipids, phospholipid-bound fatty acids and free sterols of the spongeAplysina fistularis (Pallas) formafulva (Pallas) (=Verongia thiona)(2). Isolation and structure elucidation of unprecedented.... Lipids. 2016; 16(9):631-46. DOI: 10.1007/BF02535058. View