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Co-occurrence of DON and Emerging Mycotoxins in Worldwide Finished Pig Feed and Their Combined Toxicity in Intestinal Cells

Overview
Journal Toxins (Basel)
Publisher MDPI
Specialty Toxicology
Date 2019 Dec 15
PMID 31835876
Citations 24
Authors
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Abstract

Food and feed can be naturally contaminated by several mycotoxins, and concern about the hazard of exposure to mycotoxin mixtures is increasing. In this study, more than 800 metabolites were analyzed in 524 finished pig feed samples collected worldwide. Eighty-eight percent of the samples were co-contaminated with deoxynivalenol (DON) and other regulated/emerging mycotoxins. The Top 60 emerging/regulated mycotoxins co-occurring with DON in pig feed shows that 48%, 13%, 8% and 12% are produced by Fusarium, Aspergillus, Penicillium and Alternaria species, respectively. Then, the individual and combined toxicity of DON and the 10 most prevalent emerging mycotoxins (brevianamide F, cyclo-(L-Pro-L-Tyr), tryptophol, enniatins A1, B, B1, emodin, aurofusarin, beauvericin and apicidin) was measured at three ratios corresponding to pig feed contamination. Toxicity was assessed by measuring the viability of intestinal porcine epithelial cells, IPEC-1, at 48-h. BRV-F, Cyclo and TRPT did not alter cell viability. The other metabolites were ranked in the following order of toxicity: apicidin > enniatin A1 > DON > beauvericin > enniatin B > enniatin B1 > emodin > aurofusarin. In most of the mixtures, combined toxicity was similar to the toxicity of DON alone. In terms of pig health, these results demonstrate that the co-occurrence of emerging mycotoxins that we tested with DON does not exacerbate toxicity.

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References
1.
Streit E, Schatzmayr G, Tassis P, Tzika E, Marin D, Taranu I . Current situation of mycotoxin contamination and co-occurrence in animal feed--focus on Europe. Toxins (Basel). 2012; 4(10):788-809. PMC: 3496989. DOI: 10.3390/toxins4100788. View

2.
Alassane-Kpembi I, Puel O, Pinton P, Cossalter A, Chou T, Oswald I . Co-exposure to low doses of the food contaminants deoxynivalenol and nivalenol has a synergistic inflammatory effect on intestinal explants. Arch Toxicol. 2016; 91(7):2677-2687. DOI: 10.1007/s00204-016-1902-9. View

3.
Taranu I, Marina D, Burlacu R, Pinton P, Damian V, Oswald I . Comparative aspects of in vitro proliferation of human and porcine lymphocytes exposed to mycotoxins. Arch Anim Nutr. 2010; 64(5):383-93. DOI: 10.1080/1745039X.2010.492140. View

4.
Bauden M, Tassidis H, Ansari D . In vitro cytotoxicity evaluation of HDAC inhibitor Apicidin in pancreatic carcinoma cells subsequent time and dose dependent treatment. Toxicol Lett. 2015; 236(1):8-15. DOI: 10.1016/j.toxlet.2015.03.017. View

5.
Bennett J, Klich M . Mycotoxins. Clin Microbiol Rev. 2003; 16(3):497-516. PMC: 164220. DOI: 10.1128/CMR.16.3.497-516.2003. View