Heterologous Expression and Functional Characterization of a GH10 Endoxylanase from Var. with Potential Biotechnological Application

Overview

Journal Biotechnol Rep (Amst)

Specialty Biotechnology

Date 2019 Dec 5

PMID 31799141

Citations 7

Authors

Josman Velasco

Bianca Oliva

Evandro Jose Mulinari

Leidy Patricia Quintero

Awana da Silva Lima

Aline Larissa Goncalves

Thiago Augusto Goncalves

Andre Damasio

Fabio Marcio Squina

Adriane Maria Ferreira Milagres

Asmaa Abdella

Mark R Wilkins

Fernando Segato

Affiliations

Soon will be listed here.

Abstract

Xylanases decrease the xylan content in pretreated biomass releasing it from hemicellulose, thus improving the accessibility of cellulose for cellulases. In this work, an endo-β-1,4-xylanase from var. (AFUMN-GH10) was successfully expressed. The structural analysis and biochemical characterization showed this AFUMN-GH10 does not contain a carbohydrate-binding module. The enzyme retained its activity in a pH range from 4.5 to 7.0, with an optimal temperature at 60 °C. AFUMN-GH10 showed the highest activity in beechwood xylan. The mode of action of AFUMN-GH10 was investigated by hydrolysis of APTS-labeled xylohexaose, which resulted in xylotriose and xylobiose as the main products. AFUMN-GH10 released 27% of residual xylan from hydrothermally-pretreated corn stover and 14% of residual xylan from hydrothermally-pretreated sugarcane bagasse. The results showed that environmentally friendly pretreatment followed by enzymatic hydrolysis with AFUMN-GH10 in low concentration is a suitable method to remove part of residual and recalcitrant hemicellulose from biomass.

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