Imaging Hair Cells Through Laser-ablated Cochlear Bone

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2019 Dec 5

PMID 31799058

Citations 1

Authors

Marilisa Romito

Ye Pu

Konstantina M Stankovic

Demetri Psaltis

Affiliations

Soon will be listed here.

Abstract

We report an innovative technique for the visualization of cells through an overlying scattering medium by combining femtosecond laser bone ablation and two-photon excitation fluorescence (TPEF) microscopy. We demonstrate the technique by imaging hair cells in an intact mouse cochlea . Intracochlear imaging is important for the assessment of hearing disorders. However, the small size of the cochlea and its encasement in the densest bone in the body present challenging obstacles, preventing the visualization of the intracochlear microanatomy using standard clinical imaging modalities. The controlled laser ablation reduces the optical scattering of the cochlear bone while the TPEF allows visualization of individual cells behind the bone. We implemented optical coherence tomography (OCT) simultaneously with the laser ablation to enhance the precision of the ablation and prevent inadvertent damage to the cells behind the bone.

Citing Articles

Two Photon Fluorescence Microscopy of the Unstained Human Cochlea Reveals Organ of Corti Cytoarchitecture.

Iyer J, Seist R, Moon I, Stankovic K Front Cell Neurosci. 2021; 15:690953.

PMID: 34421541 PMC: 8376148. DOI: 10.3389/fncel.2021.690953.

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