Long Noncoding RNA SNHG7 Contributes to Cell Proliferation, Migration, Invasion and Epithelial to Mesenchymal Transition in Non-small Cell Lung Cancer by Regulating MiR-449a/TGIF2 Axis

Overview

Journal Thorac Cancer

Specialties Oncology
Pulmonary Medicine

Date 2019 Dec 4

PMID 31793741

Citations 14

Authors

Lingling Pang

Yun Cheng

Shenchun Zou

Jie Song

Affiliations

Soon will be listed here.

Abstract

Background: Non-small cell lung cancer (NSCLC) is an intractable malignant lung cancer with high rates of metastasis and mortality. Currently, long noncoding RNA nuclear RNA host gene 7 (SNHG7) is recognized as a biomarker of multiple cancers. However, the role of SNHG7 in NSCLC requires further understanding.

Methods: The expression of SNHG7, miR-449a and TGIF2 in NSCLC tumors and cells was examined by quantitative real time polymerase chain reaction (qRT-PCR). Cell viability was measured by MTT assay. Cell migration and invasion was conducted using transwell assay. Protein expression of TGIF2, vimentin, N-cadherin and E-cadherin was detected by western blot. The interaction between miR-449a and SNHG7 or TGIF2 was determined by luciferase reporter system, RIP and RNA pull-down assay, respectively. Xenograft mice models were established by subcutaneously injecting A549 cells transfected with sh-SNHG7 and sh-control.

Results: SNHG7 expression was upregulated in NSCLC tumors and cells compared with normal tissues and cells. SNHG7 silencing repressed cell proliferation, migration, invasion and epithelial to mesenchymal transition (EMT) in NSCLC. Consistently, SNHG7 knockdown hindered tumor growth in vivo. The subsequent luciferase reporter system, RIP and RNA pull-down assay validated the interaction between miR-449a and SNHG7 or TGIF2. The rescue experiments displayed that miR-449a inhibitor counteracted SNHG7 silencing induced inhibition on proliferation, migration, invasion and EMT. Similarly, restoration of TGIF2 reversed miR-449a mediated inhibition on cell progression. In addition, the results indicated that SNHG7 could regulate cell progression by targeting miR-449a/TGIF2 axis.

Conclusion: SNHG7 contributed to cell proliferation, migration, invasion and EMT in NSCLC by upregulating TGIF2 via sponging miR-449a, representing a novel targeted therapy method for NSCLC.

Citing Articles

miR-449a: A Promising Biomarker and Therapeutic Target in Cancer and Other Diseases.

Barati T, Mirzaei Z, Ebrahimi A, Shekari Khaniani M, Mansoori Derakhshan S Cell Biochem Biophys. 2024; 82(3):1629-1650.

PMID: 38809350 DOI: 10.1007/s12013-024-01322-9.

High SNHG expression may predict a poor lung cancer prognosis based on a meta-analysis.

Shen G, Huang R, Yang S, Shen R, Gao J, Zhang Y BMC Cancer. 2023; 23(1):1243.

PMID: 38104110 PMC: 10725607. DOI: 10.1186/s12885-023-11706-4.

LncRNA SNHG11 promotes the malignant transformation of human bronchial epithelial cells induced by beryllium sulfate.

Deng W, Sun Z, Chen S, Li G, Liu X, Huang L Toxicol Res (Camb). 2022; 11(4):605-615.

PMID: 36051663 PMC: 9424703. DOI: 10.1093/toxres/tfac036.

Identification and Validation of lncRNA-SNHG17 in Lung Adenocarcinoma: A Novel Prognostic and Diagnostic Indicator.

Li X, Yuan Y, Pal M, Jiang X Front Oncol. 2022; 12:929655.

PMID: 35719962 PMC: 9198440. DOI: 10.3389/fonc.2022.929655.

Long non-coding RNA-small nucleolar RNA host gene 7 regulates inflammatory responses following spinal cord injury by regulating the microRNA-449a/TNF-α-induced protein 3-interacting protein 2 axis.

He C, Xiao J, Ye Y, Huang S, Zhong Y, Liu L Bioengineered. 2022; 13(4):10215-10226.

PMID: 35443851 PMC: 9162020. DOI: 10.1080/21655979.2022.2061294.

References

da Silva G, Bergmann A, Thuler L . Incidence and Risk Factors for Bone Metastasis in Non-Small Cell Lung Cancer. Asian Pac J Cancer Prev. 2019; 20(1):45-51. PMC: 6485556. DOI: 10.31557/APJCP.2019.20.1.45. View

Chi R, Chen X, Liu M, Zhang H, Li F, Fan X . Role of SNHG7-miR-653-5p-STAT2 feedback loop in regulating neuroblastoma progression. J Cell Physiol. 2019; 234(8):13403-13412. DOI: 10.1002/jcp.28017. View

Xu B, Zhang X, Wang S, Shi B . MiR-449a suppresses cell migration and invasion by targeting PLAGL2 in breast cancer. Pathol Res Pract. 2018; 214(5):790-795. DOI: 10.1016/j.prp.2017.12.012. View

Xue F, Liu Z, Xu J, Xu X, Chen X, Tian F . Neferine inhibits growth and migration of gastrointestinal stromal tumor cell line GIST-T1 by up-regulation of miR-449a. Biomed Pharmacother. 2018; 109:1951-1959. DOI: 10.1016/j.biopha.2018.11.029. View

Meng H, Huang Q, Zhang X, Huang J, Shen R, Zhang B . MiR-449a regulates the cell migration and invasion of human non-small cell lung carcinoma by targeting ADAM10. Onco Targets Ther. 2019; 12:3829-3838. PMC: 6529029. DOI: 10.2147/OTT.S190282. View

Sun X, Huang T, Liu Z, Sun M, Luo S . LncRNA SNHG7 contributes to tumorigenesis and progression in breast cancer by interacting with miR-34a through EMT initiation and the Notch-1 pathway. Eur J Pharmacol. 2019; 856:172407. DOI: 10.1016/j.ejphar.2019.172407. View

Shin J, Yoon J, Marwaha G . Progress in the Treatment and Outcomes for Early-Stage Non-Small Cell Lung Cancer. Lung. 2018; 196(3):351-358. DOI: 10.1007/s00408-018-0110-1. View

Zhong Z, Dong Z, Yang L, Chen X, Gong Z . MicroRNA-31-5p modulates cell cycle by targeting human mutL homolog 1 in human cancer cells. Tumour Biol. 2013; 34(3):1959-65. DOI: 10.1007/s13277-013-0741-z. View

Qi H, Wen B, Wu Q, Cheng W, Lou J, Wei J . Long noncoding RNA SNHG7 accelerates prostate cancer proliferation and cycle progression through cyclin D1 by sponging miR-503. Biomed Pharmacother. 2018; 102:326-332. DOI: 10.1016/j.biopha.2018.03.011. View

10.

Flum M, Kleemann M, Schneider H, Weis B, Fischer S, Handrick R . miR-217-5p induces apoptosis by directly targeting PRKCI, BAG3, ITGAV and MAPK1 in colorectal cancer cells. J Cell Commun Signal. 2017; 12(2):451-466. PMC: 5910322. DOI: 10.1007/s12079-017-0410-x. View

11.

Hu J, Fang Y, Cao Y, Qin R, Chen Q . miR-449a Regulates proliferation and chemosensitivity to cisplatin by targeting cyclin D1 and BCL2 in SGC7901 cells. Dig Dis Sci. 2013; 59(2):336-45. DOI: 10.1007/s10620-013-2923-3. View

12.

Li J, Lu M, Jin J, Lu X, Xu T, Jin S . miR-449a Suppresses Tamoxifen Resistance in Human Breast Cancer Cells by Targeting ADAM22. Cell Physiol Biochem. 2018; 50(1):136-149. DOI: 10.1159/000493964. View

13.

Li Y, Zeng C, Hu J, Pan Y, Shan Y, Liu B . Long non-coding RNA-SNHG7 acts as a target of miR-34a to increase GALNT7 level and regulate PI3K/Akt/mTOR pathway in colorectal cancer progression. J Hematol Oncol. 2018; 11(1):89. PMC: 6029165. DOI: 10.1186/s13045-018-0632-2. View

14.

Baek D, Lee K, Park K, Suh J, Choi S, Park K . Inhibition of miR-449a Promotes Cartilage Regeneration and Prevents Progression of Osteoarthritis in In Vivo Rat Models. Mol Ther Nucleic Acids. 2018; 13:322-333. PMC: 6197768. DOI: 10.1016/j.omtn.2018.09.015. View

15.

Gantenbein N, Bernhart E, Anders I, Golob-Schwarzl N, Krassnig S, Wodlej C . Influence of eukaryotic translation initiation factor 6 on non-small cell lung cancer development and progression. Eur J Cancer. 2018; 101:165-180. DOI: 10.1016/j.ejca.2018.07.001. View

16.

Cheng D, Fan J, Ma Y, Zhou Y, Qin K, Shi M . LncRNA SNHG7 promotes pancreatic cancer proliferation through ID4 by sponging miR-342-3p. Cell Biosci. 2019; 9:28. PMC: 6431029. DOI: 10.1186/s13578-019-0290-2. View

17.

Wang Y, Huo B, Li C, Ma G, Cao W . Knockdown of long noncoding RNA SNHG7 inhibits the proliferation and promotes apoptosis of thyroid cancer cells by downregulating BDNF. Eur Rev Med Pharmacol Sci. 2019; 23(11):4815-4821. DOI: 10.26355/eurrev_201906_18067. View

18.

She K, Yan H, Huang J, Zhou H, He J . miR-193b availability is antagonized by LncRNA-SNHG7 for FAIM2-induced tumour progression in non-small cell lung cancer. Cell Prolif. 2017; 51(1). PMC: 6528931. DOI: 10.1111/cpr.12406. View

19.

Keskin S, Kutluk A, Tas F . Prognostic and Predictive Role of Angiogenic Markers in Non- Small Cell Lung Cancer. Asian Pac J Cancer Prev. 2019; 20(3):733-736. PMC: 6825760. DOI: 10.31557/APJCP.2019.20.3.733. View

20.

Li L, Liu H, Du L, Xi P, Wang Q, Li Y . miR-449a Suppresses LDHA-Mediated Glycolysis to Enhance the Sensitivity of Non-Small Cell Lung Cancer Cells to Ionizing Radiation. Oncol Res. 2017; 26(4):547-556. PMC: 7844793. DOI: 10.3727/096504017X15016337254605. View