Generation, Purification and Engineering of Extracellular Vesicles and Their Biomedical Applications

Overview

Journal Methods

Specialty Biochemistry

Date 2019 Dec 3

PMID 31790730

Citations 31

Authors

Jin Gao

Xinyue Dong

Zhenjia Wang

Affiliations

Soon will be listed here.

Abstract

Extracellular vesicles (EVs), derived from cell membranes, demonstrate the potential to be excellent therapeutics and drug carriers. Although EVs are promising, the process to develop high-quality and scalable EVs for their translation is demanding. Within this research, we analyzed the production of EVs, their purification and their post-bioengineering, and we also discussed the biomedical applications of EVs. We focus on the developments of methods in producing EVs including biological, physical, and chemical approaches. Furthermore, we discuss the challenges and the opportunities that arose when we translated EVs in clinic. With the advancements in nanotechnology and immunology, genetically engineering EVs is a new frontier in developing new therapeutics in order to tailor to individuals and different disease stages in treatments of cancer and inflammatory diseases.

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