Kemp Eliminases of the AlleyCat Family Possess High Substrate Promiscuity

Overview

Journal ChemCatChem

Date 2019 Dec 3

PMID 31788134

Citations 3

Authors

Elizabeth A Caselle

Jennifer H Yoon

Sagar Bhattacharya

Joel J L Rempillo

Zsofia Lengyel

Areetha DSouza

Yurii S Moroz

Patricia L Tolbert

Alexander N Volkov

Marcello Forconi

Carlos A Castaneda

Olga V Makhlynets

Ivan V Korendovych

Affiliations

Soon will be listed here.

Abstract

Minimalist enzymes designed to catalyze model reactions provide useful starting points for creating catalysts for practically important chemical transformations. We have shown that Kemp eliminases of the AlleyCat family facilitate conversion of leflunomide (an immunosupressor pro-drug) to its active form teriflunomide with outstanding rate enhancement (nearly four orders of magnitude) and catalytic proficiency (more than seven orders of magnitude) without any additional optimization. This remarkable activity is achieved by properly positioning the substrate in close proximity to the catalytic glutamate with very high pK.

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