Innovative Particle Standards and Long-lived Imaging for 2D and 3D DSTORM

Overview

Journal Sci Rep

Specialty Science

Date 2019 Dec 1

PMID 31784555

Citations 5

Authors

Angelina Provost

Corentin Rousset

Laura Bourdon

Sarra Mezhoud

Emma Reungoat

Camille Fourneaux

Timothee Bresson

Marine Pauly

Nicolas Beard

Laura Possi-Tchouanlong

Boyan Grigorov

Philippe Bouvet

Jean-Jacques Diaz

Christophe Chamot

Eve-Isabelle Pecheur

Catherine Ladaviere

Marie-Therese Charreyre

Arnaud Favier

Christophe Place

Karine Monier

Affiliations

Soon will be listed here.

Abstract

Direct stochastic optical reconstruction microscopy (dSTORM), developed in the last decade, has revolutionised optical microscopy by enabling scientists to visualise objects beyond the resolution provided by conventional microscopy (200 nm). We developed an innovative method based on blinking particle standards and conditions for long-lived imaging over several weeks. Stable localisation precisions within the 10 nm-range were achieved for single virions and in cellulo 2D imaging of centrosomes, as well as their reliable reconstruction in 3D dSTORM.

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