Super-resolution Architecture of Mammalian Centriole Distal Appendages Reveals Distinct Blade and Matrix Functional Components

Overview

Journal Nat Commun

Specialty Biology

Date 2018 May 24

PMID 29789620

Citations 97

Authors

T Tony Yang

Weng Man Chong

Won-Jing Wang

Gregory Mazo

Barbara Tanos

Zhengmin Chen

Thi Minh Nguyet Tran

Yi-De Chen

Rueyhung Roc Weng

Chia-En Huang

Wann-Neng Jane

Meng-Fu Bryan Tsou

Jung-Chi Liao

Affiliations

Soon will be listed here.

Abstract

Distal appendages (DAPs) are nanoscale, pinwheel-like structures protruding from the distal end of the centriole that mediate membrane docking during ciliogenesis, marking the cilia base around the ciliary gate. Here we determine a super-resolved multiplex of 16 centriole-distal-end components. Surprisingly, rather than pinwheels, intact DAPs exhibit a cone-shaped architecture with components filling the space between each pinwheel blade, a new structural element we term the distal appendage matrix (DAM). Specifically, CEP83, CEP89, SCLT1, and CEP164 form the backbone of pinwheel blades, with CEP83 confined at the root and CEP164 extending to the tip near the membrane-docking site. By contrast, FBF1 marks the distal end of the DAM near the ciliary membrane. Strikingly, unlike CEP164, which is essential for ciliogenesis, FBF1 is required for ciliary gating of transmembrane proteins, revealing DAPs as an essential component of the ciliary gate. Our findings redefine both the structure and function of DAPs.

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