Comparative Pharmacokinetic Studies of Four Ginsenosides in Rat Plasma by UPLC-MS/MS After Oral Administration of - and Extracts

Overview

Journal J Anal Methods Chem

Publisher Wiley

Specialty Chemistry

Date 2019 Nov 30

PMID 31781472

Citations 3

Authors

Hailong Xie

Dongxue Wang

Wenjun Zhang

Xinjia Yan

Ying Zhao

Affiliations

Soon will be listed here.

Abstract

(PQ) and (AG) are drug target pairs in traditional Chinese medicine (TCM), which are used to treat age-related diseases. In the present study, we simultaneously determined the contents of four main bioactive ginsenosides (Rb, Rb, Rd, and Re) in rat plasma using an ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method. Plasma specimens were purified by using the solid-phase extraction procedure, and separation was performed on Waters ACQUITY UPLC BEH C (100 mm × 2.1 mm, 1.7 m) in multiple reaction monitoring (MRM) mode and negative electrospray ionization (ESI) mode. The established UPLC-MS/MS method showed good linear correlation ( ≥ 0.9978), stability (-11.93 to 12.11%), precision (RSD < 14.63%), and recovery (76.43%-95.20%). The lower limit of quantification was 3.6 ng/mL for Rb, 1.6 ng/mL for Rb, 1.2 ng/mL for Rd, and 2.5 ng/mL for Re. This validated method was successfully employed to investigate the pharmacokinetics of the four ginsenosides in rat plasma after oral administration of PQ-AG and PQ extracts. The results revealed the pharmacokinetic profiles of PQ-AG drug pair and clarified that AG played a critical role in stimulating the absorption of active ginsenosides in PQ. Collectively, our findings provided valid and reliable evidence for the rational use of PQ-AG in clinical practice.

Citing Articles

Methods on improvements of the poor oral bioavailability of ginsenosides: Pre-processing, structural modification, drug combination, and micro- or nano- delivery system.

Hu Q, Hong H, Zhang Z, Feng H, Luo T, Li J J Ginseng Res. 2023; 47(6):694-705.

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Hippocampal proteins discovery of Panax quinquefolius and Acorus gramineus ameliorating cognitive impairment in diabetic rats.

Huan S, Yang Y, Wang D, Zhao Y, Zhang X, Zheng Y Psychopharmacology (Berl). 2023; 240(8):1759-1773.

PMID: 37306736 DOI: 10.1007/s00213-023-06393-9.

Ginsenoside Rb2: A review of pharmacokinetics and pharmacological effects.

Miao L, Yang Y, Li Z, Fang Z, Zhang Y, Han C J Ginseng Res. 2022; 46(2):206-213.

PMID: 35509822 PMC: 9058830. DOI: 10.1016/j.jgr.2021.11.007.

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