LncRNA MALAT1 Acts As a MiR-125a-3p Sponge to Regulate FOXM1 Expression and Promote Hepatocellular Carcinoma Progression

Overview

Journal J Cancer

Specialty Oncology

Date 2019 Nov 29

PMID 31777593

Citations 27

Authors

ShiHai Liu

Jing Qiu

Guifang He

Ye Liang

Liping Wang

Changchang Liu

Huazheng Pan

Affiliations

Soon will be listed here.

Abstract

Hepatocellular carcinoma (HCC) is a prominent cancer type, with long non-coding RNAs (lncRNAs) being known to be relevant to its progression. We therefore investigated how a particular lncRNA known as the metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) was associated with HCC. Quantitative reverse transcriptase PCR (qPCR) was used to assess expression of MALAT1, Forkhead Box M1 (FOXM1) and miR-125a-3p in HCC tissue samples. How MALAT1 regulates HCC proliferation and metastasis was assessed through appropriate assays. FOXM1 was identified as a miR-125a-3p target using luciferase assays, and how MALAT1/miR-125a-3p alter FOXM1 expression was explored via qPCR and Western blotting. HCC tissues exhibited MALAT1 upregulation. miR-125a-3p targeted FOXM1 and could be regulated by MALAT1. MALAT1 knockdown disrupted proliferation and invasion, whereas miR-125a-3p knockdown partially reversed this phenotype. These results indicate that MALAT1 modulates FOXM1 expression via being a miR-125a-3p sponge, thus promoting HCC progression.

Citing Articles

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