Integrative Analysis of Expression Profiles of MicroRNAs and MRNAs in Treatment of Acute Myocardial Infarction with Compound Longmaining Decoction

Overview

Journal Med Sci Monit

Specialties General Medicine
Pathology

Date 2019 Nov 29

PMID 31776323

Citations 1

Authors

Diaodiao Bu

Zhuo Su

Mei Meng

Changli Wang

Affiliations

Soon will be listed here.

Abstract

BACKGROUND This study identified microRNAs (miRNAs) and mRNAs associated with Compound Longmaining (CLMN) treatment of acute myocardial infarction (AMI). Our results provide a theoretical framework to guide AMI treatment and improve myocardial injury. MATERIAL AND METHODS The myocardial tissues of the sham operation group (S), the model group (M), and the CLMN treatment group (T) were obtained. The mRNA and miRNA expression profiles were identified using RNA-sequencing analysis. The sequencing results were verified by quantitative real-time PCR (qRT-PCR). Bioinformatics was used to predict the function of differentially expressed genes (DEGs) and related signal transduction pathways. The target genes of miRNAs were predicted by software analysis, and the relationship between miRNA and mRNA was studied by network analysis. RESULTS RNA-sequencing revealed 22 differentially expressed miRNAs (DEMs) and 76 DEGs in myocardial tissue. Six DEMs and 9 DEGs were randomly selected for qRT-PCR validation, and corroborating results were obtained. The results of Gene ontology (GO) showed that DEGs participated in different biological processes. Through the combined analysis of miRNAs and mRNAs expression, it was confirmed that a single miRNA is involved in the regulation of multiple genes, and also multiple miRNAs can target one gene. CONCLUSIONS The analysis based on the miRNA-mRNA network can not only help to elucidate the potential molecular mechanism of CLMN treatment of AMI, but can also help in identifying novel therapeutic targets.

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References

Ganesan J, Ramanujam D, Sassi Y, Ahles A, Jentzsch C, Werfel S . MiR-378 controls cardiac hypertrophy by combined repression of mitogen-activated protein kinase pathway factors. Circulation. 2013; 127(21):2097-106. DOI: 10.1161/CIRCULATIONAHA.112.000882. View

Fang J, Song X, Tian J, Chen H, Li D, Wang J . Overexpression of microRNA-378 attenuates ischemia-induced apoptosis by inhibiting caspase-3 expression in cardiac myocytes. Apoptosis. 2011; 17(4):410-23. DOI: 10.1007/s10495-011-0683-0. View

Passino C, Barison A, Vergaro G, Gabutti A, Borrelli C, Emdin M . Markers of fibrosis, inflammation, and remodeling pathways in heart failure. Clin Chim Acta. 2014; 443:29-38. DOI: 10.1016/j.cca.2014.09.006. View

Monzo M, Navarro A, Bandres E, Artells R, Moreno I, Gel B . Overlapping expression of microRNAs in human embryonic colon and colorectal cancer. Cell Res. 2008; 18(8):823-33. DOI: 10.1038/cr.2008.81. View

Zhang X, Lu Z, Liu L . Coronary heart disease in China. Heart. 2008; 94(9):1126-31. DOI: 10.1136/hrt.2007.132423. View

Gidlof O, Smith J, Miyazu K, Gilje P, Spencer A, Blomquist S . Circulating cardio-enriched microRNAs are associated with long-term prognosis following myocardial infarction. BMC Cardiovasc Disord. 2013; 13:12. PMC: 3598930. DOI: 10.1186/1471-2261-13-12. View

Lin W, Lo L, Chen H, Lai C, Yamada S, Liu S . Sleep-related changes in cardiovascular autonomic regulation in left coronary artery ligation rats: Neural mechanism facilitating arrhythmia after myocardial infarction. Int J Cardiol. 2016; 225:65-72. DOI: 10.1016/j.ijcard.2016.09.121. View

Cheng Z, DiMichele L, Hakim Z, Rojas M, Mack C, Taylor J . Targeted focal adhesion kinase activation in cardiomyocytes protects the heart from ischemia/reperfusion injury. Arterioscler Thromb Vasc Biol. 2012; 32(4):924-33. PMC: 4237311. DOI: 10.1161/ATVBAHA.112.245134. View

Li Y, Xu J, Chen H, Bai J, Li S, Zhao Z . Comprehensive analysis of the functional microRNA-mRNA regulatory network identifies miRNA signatures associated with glioma malignant progression. Nucleic Acids Res. 2013; 41(22):e203. PMC: 3905890. DOI: 10.1093/nar/gkt1054. View

10.

Wang C, Bai X, Liu S, Wang J, Su Z, Zhang W . RNA-seq based transcriptome analysis of the protective effect of compound longmaining decoction on acute myocardial infarction. J Pharm Biomed Anal. 2018; 158:339-345. DOI: 10.1016/j.jpba.2018.06.016. View

11.

Martos R, Baugh J, Ledwidge M, OLoughlin C, Conlon C, Patle A . Diastolic heart failure: evidence of increased myocardial collagen turnover linked to diastolic dysfunction. Circulation. 2007; 115(7):888-95. DOI: 10.1161/CIRCULATIONAHA.106.638569. View

12.

Wagsater D, Bjork H, Zhu C, Bjorkegren J, Valen G, Hamsten A . ADAMTS-4 and -8 are inflammatory regulated enzymes expressed in macrophage-rich areas of human atherosclerotic plaques. Atherosclerosis. 2007; 196(2):514-22. DOI: 10.1016/j.atherosclerosis.2007.05.018. View

13.

Morrison A, Li J . PPAR-γ and AMPK--advantageous targets for myocardial ischemia/reperfusion therapy. Biochem Pharmacol. 2011; 82(3):195-200. DOI: 10.1016/j.bcp.2011.04.004. View

14.

Du J, Cong B, Yu Q, Wang H, Wang L, Wang C . Upregulation of microRNA-22 contributes to myocardial ischemia-reperfusion injury by interfering with the mitochondrial function. Free Radic Biol Med. 2016; 96:406-17. DOI: 10.1016/j.freeradbiomed.2016.05.006. View

15.

Lau N, Lim L, WEINSTEIN E, Bartel D . An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans. Science. 2001; 294(5543):858-62. DOI: 10.1126/science.1065062. View

16.

Anders S, Huber W . Differential expression analysis for sequence count data. Genome Biol. 2010; 11(10):R106. PMC: 3218662. DOI: 10.1186/gb-2010-11-10-r106. View

17.

White H, Chew D . Acute myocardial infarction. Lancet. 2008; 372(9638):570-84. DOI: 10.1016/S0140-6736(08)61237-4. View

18.

Li Y, McTiernan C, Feldman A . Interplay of matrix metalloproteinases, tissue inhibitors of metalloproteinases and their regulators in cardiac matrix remodeling. Cardiovasc Res. 2000; 46(2):214-24. DOI: 10.1016/s0008-6363(00)00003-1. View

19.

Duisters R, Tijsen A, Schroen B, Leenders J, Lentink V, van der Made I . miR-133 and miR-30 regulate connective tissue growth factor: implications for a role of microRNAs in myocardial matrix remodeling. Circ Res. 2008; 104(2):170-8, 6p following 178. DOI: 10.1161/CIRCRESAHA.108.182535. View

20.

Gurha P, Abreu-Goodger C, Wang T, Ramirez M, Drumond A, van Dongen S . Targeted deletion of microRNA-22 promotes stress-induced cardiac dilation and contractile dysfunction. Circulation. 2012; 125(22):2751-61. PMC: 3503489. DOI: 10.1161/CIRCULATIONAHA.111.044354. View