» Articles » PMID: 31772319

First Molecular Detection of Toxoplasma Gondii in Vegetable Samples in China Using Qualitative, Quantitative Real-time PCR and Multilocus Genotyping

Overview
Journal Sci Rep
Specialty Science
Date 2019 Nov 28
PMID 31772319
Citations 11
Authors
Affiliations
Soon will be listed here.
Abstract

Toxoplasma gondii infection is becoming increasing problem in China but there is no data concerning contamination of vegetables intended for consumption with this parasite. The aim of the present study was to investigate fresh vegetables originated from open markets located in the Xining City, the Qinghai-Tibet Plateau (QTP), P.R. China for their contamination with T. gondii. A total of 279 fresh vegetable samples were collected and analysed using real-time PCR assay targeting B1 gene and multilocus genotyping. T. gondii DNA was found in 10 (3.6%) samples tested; eight of them represented T. gondii type I and remaining two T. gondii type II. The approximate level of contamination of positive vegetables samples, estimated based on quantitative real-time PCR (qPCR), ranged between less than one and 27000 T. gondii oocysts per sample, with majority not exceeding several oocysts per sample. The results of the study confirmed that T. gondii is present in vegetables offered in open markets in the Qinghai province, P.R. China; eating them unwashed and raw may therefore pose a threat to consumers. This is the first investigation describing T. gondii detection in fresh vegetables intended for consumption collected from the territory of P.R. China using sensitive molecular tools.

Citing Articles

Prevalence of IgG Antibodies and Associated Risk Factors in Psychiatric Patients from Western Romania: A Cross-Sectional Study.

Grada S, Mihu A, Oatis D, Susan M, Lupu M, Olariu T Microorganisms. 2024; 12(1).

PMID: 38257998 PMC: 10820121. DOI: 10.3390/microorganisms12010172.


Protozoan parasites and free-living amoebae contamination in organic leafy green vegetables and strawberries from Spain.

Moreno-Mesonero L, Soler L, Amoros I, Moreno Y, Ferrus M, Alonso J Food Waterborne Parasitol. 2023; 32:e00200.

PMID: 37405064 PMC: 10316001. DOI: 10.1016/j.fawpar.2023.e00200.


Serotypes in Italian and Foreign Populations: A Cross-Sectional Study Using a Homemade ELISA Test.

Caldrer S, Vola A, Ferrari G, Ursini T, Mazzi C, Meroni V Microorganisms. 2022; 10(8).

PMID: 36013995 PMC: 9415598. DOI: 10.3390/microorganisms10081577.


in Foods: Prevalence, Control, and Safety.

Marin-Garcia P, Planas N, Llobat L Foods. 2022; 11(16).

PMID: 36010541 PMC: 9407268. DOI: 10.3390/foods11162542.


Molecular Characterization of in Cats and Its Zoonotic Potential for Public Health Significance.

Hafeez M, Mehdi M, Aslam F, Ashraf K, Aleem M, Khalid A Pathogens. 2022; 11(4).

PMID: 35456112 PMC: 9026455. DOI: 10.3390/pathogens11040437.


References
1.
Wells B, Shaw H, Innocent G, Guido S, Hotchkiss E, Parigi M . Molecular detection of Toxoplasma gondii in water samples from Scotland and a comparison between the 529bp real-time PCR and ITS1 nested PCR. Water Res. 2015; 87:175-81. DOI: 10.1016/j.watres.2015.09.015. View

2.
Ding H, Gao Y, Deng Y, Lamberton P, Lu D . A systematic review and meta-analysis of the seroprevalence of Toxoplasma gondii in cats in mainland China. Parasit Vectors. 2017; 10(1):27. PMC: 5237326. DOI: 10.1186/s13071-017-1970-6. View

3.
Alvarado-Esquivel C, Estrada-Martinez S, Liesenfeld O . Toxoplasma gondii infection in workers occupationally exposed to unwashed raw fruits and vegetables: a case control seroprevalence study. Parasit Vectors. 2011; 4:235. PMC: 3259050. DOI: 10.1186/1756-3305-4-235. View

4.
Kapperud G, Jenum P, Stray-Pedersen B, Melby K, Eskild A, Eng J . Risk factors for Toxoplasma gondii infection in pregnancy. Results of a prospective case-control study in Norway. Am J Epidemiol. 1996; 144(4):405-12. DOI: 10.1093/oxfordjournals.aje.a008942. View

5.
Gallas-Lindemann C, Sotiriadou I, Mahmoodi M, Karanis P . Detection of Toxoplasma gondii oocysts in different water resources by Loop Mediated Isothermal Amplification (LAMP). Acta Trop. 2012; 125(2):231-6. DOI: 10.1016/j.actatropica.2012.10.007. View