All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures

Overview

Journal iScience

Publisher Cell Press

Date 2019 Nov 26

PMID 31765994

Citations 4

Authors

Chiao Huang

Chu-Yi Tai

Kai-Ping Yang

Wei-Kun Chang

Kuo-Jen Hsu

Ching-Chun Hsiao

Shun-Chi Wu

Yen-Yin Lin

Ann-Shyn Chiang

Shi-Wei Chu

Affiliations

Soon will be listed here.

Abstract

All-optical physiology (AOP) manipulates and reports neuronal activities with light, allowing for interrogation of neuronal functional connections with high spatiotemporal resolution. However, contemporary high-speed AOP platforms are limited to single-depth or discrete multi-plane recordings that are not suitable for studying functional connections among densely packed small neurons, such as neurons in Drosophila brains. Here, we constructed a 3D AOP platform by incorporating single-photon point stimulation and two-photon high-speed volumetric recordings with a tunable acoustic gradient-index (TAG) lens. We demonstrated the platform effectiveness by studying the anterior visual pathway (AVP) of Drosophila. We achieved functional observation of spatiotemporal coding and the strengths of calcium-sensitive connections between anterior optic tubercle (AOTU) sub-compartments and >70 tightly assembled 2-μm bulb (BU) microglomeruli in 3D coordinates with a single trial. Our work aids the establishment of in vivo 3D functional connectomes in neuron-dense brain areas.

Citing Articles

Connectomic reconstruction predicts visual features used for navigation.

Garner D, Kind E, Lai J, Nern A, Zhao A, Houghton L Nature. 2024; 634(8032):181-190.

PMID: 39358517 PMC: 11446847. DOI: 10.1038/s41586-024-07967-z.

Connectomic reconstruction predicts the functional organization of visual inputs to the navigation center of the brain.

Garner D, Kind E, Nern A, Houghton L, Zhao A, Sancer G bioRxiv. 2023; .

PMID: 38076786 PMC: 10705420. DOI: 10.1101/2023.11.29.569241.

High-speed laser-scanning biological microscopy using FACED.

Lai Q, Yip G, Wu J, Wong J, Lo M, Lee K Nat Protoc. 2021; 16(9):4227-4264.

PMID: 34341580 DOI: 10.1038/s41596-021-00576-4.

Dual GRIN lens two-photon endoscopy for high-speed volumetric and deep brain imaging.

Chien Y, Lin J, Yeh P, Hsu K, Tsai Y, Chen S Biomed Opt Express. 2021; 12(1):162-172.

PMID: 33659072 PMC: 7899523. DOI: 10.1364/BOE.405738.

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