Structural Analysis and Potential Anti-tumor Activity of Sporisorium Reilianum (Fries) Polysaccharide

A neutral polysaccharide WM-NP-60 was successfully isolated and purified from a phytopathogenic fungus Sporisorium reilianum (Fries). The characteristics and potential antitumor activities of WM-NP-60 were studied. WM-NP-60 was a water-soluble polysaccharide. The molecular weight of WM-NP-60 was 15.6 kDa. The main chain of WM-NP-60 was composed of β-1,6-D-Glcp and its side chains were β-1,3-D-Glcp. The side chains bound to the main chain with glycosyl groups at the C-3 positions. Gal might be attached to the backbone as a side chain or bound to the linear β-1,3-D-Glcp side chain. WM-NP-60 could inhibit the proliferation of HepG2 and SGC7901 cells in a dose-dependently manner. In addition, it was found that WM-NP-60 triggered the HepG2 and SGC7901 cell cycle arrest at the G phase and induced apoptosis of HepG2 and SGC7901 cells. Taken together, these results suggested that WM-NP-60 possessed a tumor-suppressive activity and might be regarded as a potential natural anti-tumor drug.