A Stepwise Strategy to Distinguish Menstrual Blood from Peripheral Blood by Fisher's Discriminant Function

Overview

Journal Int J Legal Med

Specialty Forensic Sciences

Date 2019 Nov 18

PMID 31734726

Citations 6

Authors

Hongxia He

Anquan Ji

Yixia Zhao

Na Han

Sheng Hu

Qinglan Kong

Li Jiang

Jian Ye

Yao Liu

Qifan Sun

Affiliations

Soon will be listed here.

Abstract

Blood samples are the most common and important biological samples found at crime scenes, and distinguishing peripheral blood and menstrual blood samples is crucial for solving criminal cases. MicroRNAs (miRNAs) are important molecules with strong tissue specificity that can be used in forensic fields to identify the tissue properties of body fluid samples. In this study, the relative expression levels of four different miRNAs (miR-451, miR-205, miR-214 and miR-203) were analysed by real-time PCR, with 200 samples from 5 different body fluids, including two kinds of blood samples (peripheral blood and menstrual blood) and three kinds of non-blood samples (saliva, semen and vaginal secretion). Then, a strategy for identifying menstrual and peripheral blood based on Fisher's discriminant function and the relative expression of multiple miRNAs was established. Two sets of functions were used: Z1 and Z2 were used to distinguish blood samples from non-blood samples, and Y1 and Y2 were used to distinguish peripheral blood from menstrual blood. A 100% accuracy rate was achieved when 50 test samples were used. Ten samples were used to test the sensitivity of the method, and 10 ng or more of total RNA from peripheral blood samples and 10 pg or more of total RNA from menstrual blood samples were sufficient for this method. The results provide a scientific reference to address the difficult forensic problem of distinguishing menstrual blood from peripheral blood.

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References

Hanson E, Lubenow H, Ballantyne J . Identification of forensically relevant body fluids using a panel of differentially expressed microRNAs. Anal Biochem. 2009; 387(2):303-14. DOI: 10.1016/j.ab.2009.01.037. View

Liang Y, Ridzon D, Wong L, Chen C . Characterization of microRNA expression profiles in normal human tissues. BMC Genomics. 2007; 8:166. PMC: 1904203. DOI: 10.1186/1471-2164-8-166. View

Wang Z, Zhang J, Wei W, Zhou D, Luo H, Chen X . Identification of Saliva Using MicroRNA Biomarkers for Forensic Purpose. J Forensic Sci. 2015; 60(3):702-6. DOI: 10.1111/1556-4029.12730. View

An J, Shin K, Yang W, Lee H . Body fluid identification in forensics. BMB Rep. 2012; 45(10):545-53. DOI: 10.5483/bmbrep.2012.45.10.206. View

Jakubowska J, Maciejewska A, Bielawski K, Pawlowski R . mRNA heptaplex protocol for distinguishing between menstrual and peripheral blood. Forensic Sci Int Genet. 2014; 13:53-60. DOI: 10.1016/j.fsigen.2014.07.006. View

Qin A, Zhang X, Liu L, Yu J, Li H, Wang S . MiR-205 in cancer: an angel or a devil?. Eur J Cell Biol. 2013; 92(2):54-60. DOI: 10.1016/j.ejcb.2012.11.002. View

Sauer E, Reinke A, Courts C . Differentiation of five body fluids from forensic samples by expression analysis of four microRNAs using quantitative PCR. Forensic Sci Int Genet. 2016; 22:89-99. DOI: 10.1016/j.fsigen.2016.01.018. View

Uchimoto M, Beasley E, Coult N, Omelia E, World D, Williams G . Considering the effect of stem-loop reverse transcription and real-time PCR analysis of blood and saliva specific microRNA markers upon mixed body fluid stains. Forensic Sci Int Genet. 2013; 7(4):418-21. DOI: 10.1016/j.fsigen.2013.04.006. View

Silva S, Lopes C, Teixeira A, Carneiro de Sousa M, Medeiros R . Forensic miRNA: potential biomarker for body fluids?. Forensic Sci Int Genet. 2014; 14:1-10. DOI: 10.1016/j.fsigen.2014.09.002. View

10.

Courts C, Madea B . Specific micro-RNA signatures for the detection of saliva and blood in forensic body-fluid identification. J Forensic Sci. 2011; 56(6):1464-70. DOI: 10.1111/j.1556-4029.2011.01894.x. View

11.

van der Meer D, Uchimoto M, Williams G . Simultaneous analysis of micro-RNA and DNA for determining the body fluid origin of DNA profiles. J Forensic Sci. 2013; 58(4):967-71. DOI: 10.1111/1556-4029.12160. View

12.

Hanson E, Mirza M, Rekab K, Ballantyne J . The identification of menstrual blood in forensic samples by logistic regression modeling of miRNA expression. Electrophoresis. 2014; 35(21-22):3087-95. DOI: 10.1002/elps.201400171. View

13.

Sonkoly E, Wei T, Janson P, Saaf A, Lundeberg L, Tengvall-Linder M . MicroRNAs: novel regulators involved in the pathogenesis of psoriasis?. PLoS One. 2007; 2(7):e610. PMC: 1905940. DOI: 10.1371/journal.pone.0000610. View

14.

O Leary K, Glynn C . Investigating the Isolation and Amplification of microRNAs for Forensic Body Fluid Identification. Microrna. 2018; 7(3):187-194. DOI: 10.2174/2211536607666180430153821. View

15.

Li Z, Bai P, Peng D, Wang H, Guo Y, Jiang Y . Screening and confirmation of microRNA markers for distinguishing between menstrual and peripheral blood. Forensic Sci Int Genet. 2017; 30:24-33. DOI: 10.1016/j.fsigen.2017.05.012. View

16.

Tian H, Lv M, Li Z, Peng D, Tan Y, Wang H . Semen-specific miRNAs: Suitable for the distinction of infertile semen in the body fluid identification?. Forensic Sci Int Genet. 2018; 33:161-167. DOI: 10.1016/j.fsigen.2017.12.010. View

17.

Lee R, Feinbaum R, Ambros V . The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell. 1993; 75(5):843-54. DOI: 10.1016/0092-8674(93)90529-y. View

18.

Grabmuller M, Madea B, Courts C . Comparative evaluation of different extraction and quantification methods for forensic RNA analysis. Forensic Sci Int Genet. 2015; 16:195-202. DOI: 10.1016/j.fsigen.2015.01.006. View

19.

Li Y, Zhang J, Wei W, Wang Z, Prinz M, Hou Y . A strategy for co-analysis of microRNAs and DNA. Forensic Sci Int Genet. 2014; 12:24-9. DOI: 10.1016/j.fsigen.2014.04.011. View

20.

Mayes C, Seashols-Williams S, Hughes-Stamm S . A capillary electrophoresis method for identifying forensically relevant body fluids using miRNAs. Leg Med (Tokyo). 2017; 30:1-4. DOI: 10.1016/j.legalmed.2017.10.013. View