POTEE Drives Colorectal Cancer Development Via Regulating SPHK1/p65 Signaling

Overview

Journal Cell Death Dis

Specialties Cell Biology
General Medicine

Date 2019 Nov 15

PMID 31723122

Citations 18

Authors

Zhiyong Shen

Xiaochuang Feng

Yuan Fang

Yongsheng Li

Zhenkang Li

Yizhi Zhan

Mingdao Lin

Guoxin Li

Yi Ding

Haijun Deng

Affiliations

Soon will be listed here.

Abstract

Aberrant gene expression plays critical roles in the development of colorectal cancer (CRC). Here we show that POTEE, which was identified as a member E of POTE ankyrin domain family, was significantly upregulated in colorectal tumors and predicted poor overall survival of CRC patients. In CRC cells, POTEE could act as an oncogene and could promote cell growth, cell-cycle progression, inhibit apoptosis, and elevates xenograft tumor growth. Mechanically, we used microarray analysis and identified a POTEE/SPHK1/p65 signaling axis, which affected the biological functions of CRC cells. Further evaluation showed that overexpression of POTEE could increase the protein expression of SPHK1, followed by promoting the phosphorylation and activation of p65 protein. Altogether, our findings suggested a POTEE/SPHK1/p65 signaling axis could promote colorectal tumorigenesis and POTEE might potentially serve as a novel biomarker for the diagnosis and an intervention of colorectal cancer.

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References

Sung J, Ng S, Chan F, Chiu H, Kim H, Matsuda T . An updated Asia Pacific Consensus Recommendations on colorectal cancer screening. Gut. 2014; 64(1):121-32. DOI: 10.1136/gutjnl-2013-306503. View

Tang W, Zhou W, Xiang L, Wu X, Zhang P, Wang J . The p300/YY1/miR-500a-5p/HDAC2 signalling axis regulates cell proliferation in human colorectal cancer. Nat Commun. 2019; 10(1):663. PMC: 6368584. DOI: 10.1038/s41467-018-08225-3. View

Bera T, Huynh N, Maeda H, Sathyanarayana B, Lee B, Pastan I . Five POTE paralogs and their splice variants are expressed in human prostate and encode proteins of different lengths. Gene. 2004; 337:45-53. DOI: 10.1016/j.gene.2004.05.009. View

Schmitz M, Kracht M . Cyclin-Dependent Kinases as Coregulators of Inflammatory Gene Expression. Trends Pharmacol Sci. 2016; 37(2):101-113. DOI: 10.1016/j.tips.2015.10.004. View

Shi W, Zhai C, Feng W, Wang J, Zhu Y, Li S . Resveratrol inhibits monocrotaline-induced pulmonary arterial remodeling by suppression of SphK1-mediated NF-κB activation. Life Sci. 2018; 210:140-149. DOI: 10.1016/j.lfs.2018.08.071. View

Bera T, Zimonjic D, Popescu N, Sathyanarayana B, Kumar V, Lee B . POTE, a highly homologous gene family located on numerous chromosomes and expressed in prostate, ovary, testis, placenta, and prostate cancer. Proc Natl Acad Sci U S A. 2002; 99(26):16975-80. PMC: 139254. DOI: 10.1073/pnas.262655399. View

Speirs M, Swensen A, Chan T, Jones P, Holman J, Harris M . Imbalanced sphingolipid signaling is maintained as a core proponent of a cancerous phenotype in spite of metabolic pressure and epigenetic drift. Oncotarget. 2019; 10(4):449-479. PMC: 6355186. DOI: 10.18632/oncotarget.26533. View

Siegel R, Miller K, Fedewa S, Ahnen D, Meester R, Barzi A . Colorectal cancer statistics, 2017. CA Cancer J Clin. 2017; 67(3):177-193. DOI: 10.3322/caac.21395. View

Luo X, Zhang X, Gan L, Zhou C, Zhao T, Zeng T . The outer membrane protein Tp92 of Treponema pallidum induces human mononuclear cell death and IL-8 secretion. J Cell Mol Med. 2019; 22(12):6039-6054. PMC: 6237608. DOI: 10.1111/jcmm.13879. View

10.

Johnson K, Johnson K, Crellin H, Ogretmen B, Boylan A, Harley R . Immunohistochemical distribution of sphingosine kinase 1 in normal and tumor lung tissue. J Histochem Cytochem. 2005; 53(9):1159-66. DOI: 10.1369/jhc.4A6606.2005. View

11.

Misawa A, Takayama K, Fujimura T, Homma Y, Suzuki Y, Inoue S . Androgen-induced lncRNA POTEF-AS1 regulates apoptosis-related pathway to facilitate cell survival in prostate cancer cells. Cancer Sci. 2016; 108(3):373-379. PMC: 5378265. DOI: 10.1111/cas.13151. View

12.

Wang Y, Xu H, Jiao H, Wang S, Xiao Z, Zhao Y . STX2 promotes colorectal cancer metastasis through a positive feedback loop that activates the NF-κB pathway. Cell Death Dis. 2018; 9(6):664. PMC: 5981218. DOI: 10.1038/s41419-018-0675-x. View

13.

Fang Y, Shen Z, Zhan Y, Feng X, Chen K, Li Y . CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis. Nat Commun. 2019; 10(1):3981. PMC: 6726635. DOI: 10.1038/s41467-019-11662-3. View

14.

Cine N, Baykal A, Sunnetci D, Canturk Z, Serhatli M, Savli H . Identification of ApoA1, HPX and POTEE genes by omic analysis in breast cancer. Oncol Rep. 2014; 32(3):1078-86. DOI: 10.3892/or.2014.3277. View

15.

Chen T, Li J, Xu M, Zhao Q, Hou Y, Yao L . PKCε phosphorylates MIIP and promotes colorectal cancer metastasis through inhibition of RelA deacetylation. Nat Commun. 2017; 8(1):939. PMC: 5643311. DOI: 10.1038/s41467-017-01024-2. View

16.

Chen Q, Shi P, Wang Y, Zou D, Wu X, Wang D . GSDMB promotes non-canonical pyroptosis by enhancing caspase-4 activity. J Mol Cell Biol. 2018; 11(6):496-508. PMC: 6734491. DOI: 10.1093/jmcb/mjy056. View

17.

Heffernan-Stroud L, Helke K, Jenkins R, De Costa A, Hannun Y, Obeid L . Defining a role for sphingosine kinase 1 in p53-dependent tumors. Oncogene. 2011; 31(9):1166-75. PMC: 3278571. DOI: 10.1038/onc.2011.302. View

18.

Hisano Y, Hla T . Bioactive lysolipids in cancer and angiogenesis. Pharmacol Ther. 2018; 193:91-98. PMC: 6309747. DOI: 10.1016/j.pharmthera.2018.07.006. View

19.

Bera T, Saint Fleur A, Lee Y, Kydd A, Hahn Y, Popescu N . POTE paralogs are induced and differentially expressed in many cancers. Cancer Res. 2006; 66(1):52-6. DOI: 10.1158/0008-5472.CAN-05-3014. View

20.

Liang J, Nagahashi M, Kim E, Harikumar K, Yamada A, Huang W . Sphingosine-1-phosphate links persistent STAT3 activation, chronic intestinal inflammation, and development of colitis-associated cancer. Cancer Cell. 2013; 23(1):107-20. PMC: 3578577. DOI: 10.1016/j.ccr.2012.11.013. View