Microfluidic Cell Trap Arrays for Single Hematopoietic Stem/Progenitor Cell Behavior Analysis

Overview

Journal Proteomics

Date 2019 Nov 12

PMID 31709756

Citations 1

Authors

Xin Han

Yuan Ma

Kai Zhang

Pengchao Zhang

Ning Shao

Lidong Qin

Affiliations

Soon will be listed here.

Abstract

Hematopoietic stem/progenitor cell (HSPC) mobilization from the bone marrow to the bloodstream is a required step for blood cell renewal, and HSPC motility is a clinically relevant standard for peripheral blood stem cell transplantation. Individual HSPCs exhibit considerable heterogeneity in motility behaviors, which are subject to complex intrinsic and extrinsic regulatory mechanisms. Motility-based cell sorting is then demanded to fulfill the study of such mechanism complexity. However, due to the HSPC heterogeneity and difficulty in monitoring cell motility, such a platform is still not available. With the recent development of microfluidics technology, motility-based monitoring, sorting, collecting, and analysis of HSPC behaviors are highly possible and achievable if fluid channels and structures are correctly engineered. Here, a new design of microfluidic arrays for single-cell trapping is presented, enabling high-throughput analysis of individual HSPC motility and behavior. Using these arrays, it is observed that HSPC motility is positively correlated with CD34 asymmetric inheritance and cell differentiation. Transcriptomic analysis of HSPCs sorted according to motility reveals changes in expression of genes associated with the regulation of stem-cell maintenance. Ultimately, this novel, physical cell-sorting system can facilitate the screening of HSPC mobilization compounds and the analysis of signals driving HSPC fate decisions.

Citing Articles

A microfluidic platform enables comprehensive gene expression profiling of mouse retinal stem cells.

Coles B, Labib M, Poudineh M, Innes B, Belair-Hickey J, Gomis S Lab Chip. 2021; 21(22):4464-4476.

PMID: 34651637 PMC: 8578462. DOI: 10.1039/d1lc00790d.

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